Full Product Name
CARS siRNA (Human)
Product Synonym Names
Cysteine--tRNA ligase cytoplasmic; Cysteinyl-tRNA synthetase; CysRS
Product Gene Name
CARS sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P49589
Specificity
CARS siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CARS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CARS sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CARS sirna
siRNA to inhibit CARS expression using RNA interference
Applications Tested/Suitable for CARS sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CARS. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001014437.1
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NCBI GenBank Nucleotide #
NM_001014437.2
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UniProt Primary Accession #
P49589
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UniProt Secondary Accession #
Q53XI8; Q5HYE4; Q9HD24; Q9HD25[Other Products]
UniProt Related Accession #
P49589[Other Products]
Molecular Weight
94,638 Da
NCBI Official Full Name
cysteine--tRNA ligase, cytoplasmic isoform c
NCBI Official Synonym Full Names
cysteinyl-tRNA synthetase
NCBI Official Symbol
CARS [Similar Products]
NCBI Official Synonym Symbols
CARS1; CYSRS; MGC:11246
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NCBI Protein Information
cysteine--tRNA ligase, cytoplasmic
UniProt Protein Name
Cysteine--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names
Cysteinyl-tRNA synthetase; CysRS
Protein Family
CDP-archaeol synthase
UniProt Gene Name
CARS [Similar Products]
UniProt Synonym Gene Names
CysRS [Similar Products]
UniProt Entry Name
SYCC_HUMAN
NCBI Summary for CARS
This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010]
UniProt Comments for CARS
CARS: a class 1 aminoacyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. Two alternatively spliced isoforms are described.
Protein type: Ligase; EC 6.1.1.16; Oncoprotein; Translation
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: cytoplasm; cytosol
Molecular Function: protein binding; protein homodimerization activity; cysteine-tRNA ligase activity; metal ion binding; ATP binding; tRNA binding
Biological Process: tRNA aminoacylation for protein translation; cysteinyl-tRNA aminoacylation; gene expression
Research Articles on CARS
1. Observational study of gene-disease association. (HuGE Navigator)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
