B Raf, Monoclonal Antibody

Purified Mouse Monoclonal Antibody (Mab); FLJ95109; 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF 1; BRAF; BRAF_HUMAN; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral (v-raf) oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; NS7; oncogene BRAF1; p94; Proto-oncogene B-Raf; Proto-oncogene c-Rmil; RAFB 1; RAFB1; RMIL; Serine/threonine-protein kinase B-raf; v raf murine sarcoma viral oncogene homolog B; v raf murine sarcoma viral oncogene homolog B1; v-Raf murine sarcoma viral oncogene homolog B1

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

Mouse

Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-BRAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Cancer; Cell Biology; Signal Transduction

Western Blot (WB)

WB ~~ 1:1000

Western blot detection of B Raf in MDA-MB-468,T47D,MCF7,HCT116,C2C12,3T3 and Hela cell lysates using B Raf mouse mAb (1:1000 diluted).Predicted band size:87KDa.Observed band size:87KDa.

84437

B-Raf proto-oncogene, serine/threonine kinase

serine/threonine-protein kinase B-raf

Serine/threonine-protein kinase B-raf

BRAF1; RAFB1  [Similar Products]

BRAF_HUMAN

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]

BRAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Oncoprotein; Protein kinase, TKL; Kinase, protein; TKL group; RAF family

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: neuron projection; mitochondrion; plasma membrane; nucleus; cytosol

Molecular Function: small GTPase binding; protein serine/threonine kinase activity; identical protein binding; protein binding; MAP kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; protein heterodimerization activity; calcium ion binding; ATP binding; protein kinase activity

Biological Process: axon guidance; response to cAMP; CD4-positive, alpha beta T cell differentiation; nerve growth factor receptor signaling pathway; activation of MAPKK activity; protein heterooligomerization; somatic stem cell maintenance; glucose transport; protein amino acid phosphorylation; synaptic transmission; myeloid progenitor cell differentiation; small GTPase mediated signal transduction; positive regulation of stress fiber formation; visual learning; negative regulation of neuron apoptosis; epidermal growth factor receptor signaling pathway; positive T cell selection; positive regulation of axon regeneration; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; regulation of cell proliferation; organ morphogenesis; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; positive regulation of axonogenesis; vascular endothelial growth factor receptor signaling pathway; negative regulation of apoptosis

Disease: Noonan Syndrome 1; Noonan Syndrome 7; Lung Cancer; Cardiofaciocutaneous Syndrome 1; Leopard Syndrome 3

Stephens R.M.,et al.Mol. Cell. Biol. 12:3733-3742(1992). Albert S.,et al.Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases. Scherer S.W.,et al.Science 300:767-772(2003). Hillier L.W.,et al.Nature 424:157-164(2003). Eychene A.,et al.Oncogene 7:1657-1660(1992).

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