INPP5F, Monoclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

Mouse

This antibody recognizes human, mouse and rat INPP5F as well as INPP5b.

100ug (1mg/ml) Protein G-purified antibody in PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

This product is stable for at least one (1) year at-20 degree C.

Small volumes of anti-INPP5F antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

INPP5F (also known as ORCL, inositol polyphosphate 5-phosphatase, and Lowe oculocerebrorenal syndrome protein) is a phosphatase that converts (1) phosphatidylinositol 4,5- bisphosphate to phosphatidylinositol 4-phosphate, (2) inositol 1,4,5-trisphosphate to inositol 1,4- bisphosphate, and (3) inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. INPP5F is involved in primary cilia assembly and may also have a role in lysosomal membrane trafficking. INPP5F is expressed in brain, skeletal muscle, heart, kidney, lung, placenta, fibroblasts, retina and the retinal pigment epithelium. Defects in the INPP5F gene can lead to Lowe oculocerebrorenal syndrome and Dent disease type 2 (DD2).

Immunoblot, Immunofluorescence (IF)

Immunoblot: 1-5ug/ml. A band of ~100kDa is detected.
IF: 10ug/ml
Dilute in PBS or medium that is identical to that used in the assay system.

Detection of INPP5F in COS cells transfected with GFP-tagged INPP5F at 5ug/ml.

Detection of INPP5F in neuroblastoma cell line SK-N-BE at 10ug/ml: DAPI (blue) nuclear stain, Texas Red F actin stain, and ATTO 488 (green) INPP5F stain.

103,227 Da

OCRL, inositol polyphosphate-5-phosphatase

inositol polyphosphate 5-phosphatase OCRL-1

Inositol polyphosphate 5-phosphatase OCRL-1

INPP5F; OCRL1  [Similar Products]

This gene encodes an inositol polyphosphate 5-phosphatase. This protein is involved in regulating membrane trafficking and is located in numerous subcellular locations including the trans-Golgi network, clathrin-coated vesicles and, endosomes and the plasma membrane. This protein may also play a role in primary cilium formation. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate (PubMed:25869668, PubMed:7761412, PubMed:9430698). May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes. Involved in primary cilia assembly (PubMed:22228094, PubMed:22543976).

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