INPP5F, Monoclonal Antibody

EC 3.1.3.36 antibody; Inositol polyphosphate 5 phosphatase OCRL 1 antibody; Inositol polyphosphate 5 phosphatase OCRL1 antibody; Inositol polyphosphate 5-phosphatase OCRL-1 antibody; INPP5F antibody; LOCR antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

S166A-26

Mouse

Protein G Purified

1mg/mL (lot specific)

-20 degree C

Small volumes of anti-OCRL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background Info: Detects a 100 kDa protein. Cross-reacts with INPP5b.

Scientific Background: Inositol polyphosphate 5-phosphatase OCRL-1 (INPP5F/OCRL) is also called Lowe oculocerebrorenal syndrome protein. INPP5F is a phosphotase that converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate, as well as inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. ORCL is involved in primary cilia assembly and may also have a role in lysosomal membrane trafficking. ORCL is expressed in brain, skeletal muscle, heart, kidney, lung, placenta, fibroblasts, retina and the retinal pigment epithelium. INPP5F defects can cause Lowe oculocerebrorenal syndrome (OCRL) and Dent disease type 2 (DD2).

Neuroscience

Western Blot (WB), Immunocytochemistry (ICC)

1:1000 WB

Western Blot analysis of Monkey COS cells transfected with GFP-tagged OCRL showing detection of ~100 kDa INPP5F protein using Mouse Anti-INPP5F Monoclonal Antibody, Clone S166A-26. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged OCRL. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.

103,227 Da[Similar Products]

oculocerebrorenal syndrome of Lowe

inositol polyphosphate 5-phosphatase OCRL-1; Lowe oculocerebrorenal syndrome protein; phosphatidylinositol polyphosphate 5-phosphatase

Inositol polyphosphate 5-phosphatase OCRL-1

INPP5F; OCRL1  [Similar Products]

OCRL_HUMAN

This gene encodes a phosphatase enzyme that is involved in actin polymerization and is found in the trans-Golgi network. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. [provided by RefSeq, Jul 2008]

OCRL: Converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5- trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5- tetrakisphosphate to inositol 1,3,4-trisphosphate. May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes. Belongs to the inositol-1,4,5-trisphosphate 5- phosphatase type II family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.36; Carbohydrate Metabolism - inositol phosphate; Phosphatase (non-protein); Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: phagocytic vesicle membrane; Golgi stack; photoreceptor outer segment; clathrin-coated vesicle; early endosome membrane; early endosome; cytoplasm; plasma membrane; Golgi-associated vesicle; coated pit; trans-Golgi network; cytosol; nucleus

Molecular Function: protein binding; phosphoinositide 5-phosphatase activity; Rac GTPase binding

Biological Process: regulation of small GTPase mediated signal transduction; inositol phosphate metabolic process; in utero embryonic development; phospholipid metabolic process; small GTPase mediated signal transduction; phosphatidylinositol biosynthetic process; phosphoinositide dephosphorylation; cilium biogenesis; lipid metabolic process

Disease: Lowe Oculocerebrorenal Syndrome; Dent Disease 2

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