Protein WNT-4 (WNT4), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of WNT4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9393396 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein WNT-4 (WNT4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT4. The ELISA analytical biochemical technique of the MBS9393396 kit is based on WNT4 antibody-WNT4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

32,954 Da

Wnt family member 4

protein Wnt-4

Protein Wnt-4

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]

WNT4: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Overexpression may be associated with abnormal proliferation in human breast tissue. Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome); also called Mayer- Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls. Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL); also known as SERKAL syndrome. Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL). Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: cell surface; cytoplasm; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane

Molecular Function: frizzled binding; receptor agonist activity; transcription corepressor activity

Biological Process: adrenal gland development; androgen biosynthetic process; cell fate commitment; epithelial to mesenchymal transition; female gonad development; female sex determination; kidney development; liver development; male gonad development; negative regulation of transcription, DNA-dependent; neuron differentiation; positive regulation of aldosterone biosynthetic process; positive regulation of bone mineralization; positive regulation of collagen biosynthetic process; positive regulation of osteoblast differentiation; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin

Disease: 46,xx Sex Reversal With Dysgenesis Of Kidneys, Adrenals, And Lungs; Mullerian Aplasia And Hyperandrogenism

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