Apoptosis regulatory protein Siva (SIVA1), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of SIVA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7244544 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apoptosis regulatory protein Siva (SIVA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SIVA1. The ELISA analytical biochemical technique of the MBS7244544 kit is based on SIVA1 antibody-SIVA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SIVA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SIVA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Cell Biology

11,778 Da

SIVA1, apoptosis-inducing factor

apoptosis regulatory protein Siva; CD27-binding (Siva) protein

Apoptosis regulatory protein Siva

SIVA; CD27BP  [Similar Products]

SIVA_HUMAN

This gene encodes a protein with an important role in the apoptotic (programmed cell death) pathway induced by the CD27 antigen, a member of the tumor necrosis factor receptor (TFNR) superfamily. The CD27 antigen cytoplasmic tail binds to the N-terminus of this protein. Two alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Jul 2008]

Siva: a proapoptotic protein. Associates with CD27, a member of the tumor necrosis factor receptor family. Two alternatively spliced isoforms have been described. Isoform 1 but not isoform 2 may induce TNFRSF7/CD27 mediated apoptosis. Isoform 1 inhibits Bcl-xL anti-apoptotic activity.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: viral receptor activity; protein binding; zinc ion binding; metal ion binding; CD27 receptor binding

Biological Process: entry of virus into host cell; inhibition of NF-kappaB transcription factor; activated T cell apoptosis

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