Product Name
Collagen IX alpha3 (COL9A3), Polyclonal Antibody
Full Product Name
Collagen IX alpha3 Antibody
Product Synonym Names
Collagen IX a3 Antibody; Collagen alpha-3(IX) chain; CO9A3
Product Gene Name
anti-COL9A3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q14050
Species Reactivity
Human, Mouse
Specificity
The antibody detects endogenous levels of total Collagen IX a3 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Description
Synthesized peptide derived from internal of human Collagen IX a3.
Target Name
Collagen IX a3
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-COL9A3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-COL9A3 antibody
Structural component of hyaline cartilage and vitreous of the eye.Brewton R.G., Genomics 30:329-336(1995).Paassilta P., J. Biol. Chem. 274:22469-22475(1999).Deloukas P., Nature 414:865-871(2001).
Product Categories/Family for anti-COL9A3 antibody
Total protein Ab
Applications Tested/Suitable for anti-COL9A3 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-COL9A3 antibody
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Immunohistochemistry (IHC) of anti-COL9A3 antibody
Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using Collagen IX a3 antibody.
Immunofluorescence (IF) of anti-COL9A3 antibody
Immunofluorescence analysis of NIH/3T3 cells, using Collagen IX a3 antibody.
NCBI/Uniprot data below describe general gene information for COL9A3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001844.3
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NCBI GenBank Nucleotide #
NM_001853.3
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UniProt Primary Accession #
Q14050
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UniProt Secondary Accession #
Q13681; Q9H4G9; Q9UPE2[Other Products]
UniProt Related Accession #
Q14050[Other Products]
Molecular Weight
63,616 Da[Similar Products]
NCBI Official Full Name
collagen alpha-3(IX) chain
NCBI Official Synonym Full Names
collagen, type IX, alpha 3
NCBI Official Symbol
COL9A3 [Similar Products]
NCBI Official Synonym Symbols
IDD; MED; EDM3; DJ885L7.4.1
[Similar Products]
NCBI Protein Information
collagen alpha-3(IX) chain
UniProt Protein Name
Collagen alpha-3(IX) chain
UniProt Gene Name
COL9A3 [Similar Products]
UniProt Entry Name
CO9A3_HUMAN
NCBI Summary for COL9A3
This gene encodes one of the three alpha chains of type IX collagen, the major collagen component of hyaline cartilage. Type IX collagen, a heterotrimeric molecule, is usually found in tissues containing type II collagen, a fibrillar collagen. Mutations in this gene are associated with multiple epiphyseal dysplasia type 3. [provided by RefSeq, Jan 2010]
UniProt Comments for COL9A3
COL9A3: Structural component of hyaline cartilage and vitreous of the eye. Defects in COL9A3 are the cause of multiple epiphyseal dysplasia type 3 (EDM3); also known as multiple epiphyseal dysplasia with myopathy. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COL9A3 are a cause of susceptibility to intervertebral disk disease (IDD). A common musculo- skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Susceptibility to intervertebral disk disease, is conferred by variant p.Arg103Trp (PubMed:11308397). Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 20q13.3
Cellular Component: proteinaceous extracellular matrix; endoplasmic reticulum lumen; extracellular region
Molecular Function: extracellular matrix structural constituent conferring tensile strength
Biological Process: collagen catabolic process; extracellular matrix disassembly; axon guidance; extracellular matrix organization and biogenesis; male gonad development; female gonad development
Disease: Epiphyseal Dysplasia, Multiple, 3; Intervertebral Disc Disease
Research Articles on COL9A3
1. A novel missense mutation was identified in a family diagnosed with multiple epiphyseal dysplasia.
Precautions
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