HADH, Recombinant Protein

Hydroxyacyl-coenzyme A dehydrogenase mitochondrial; HAD; HADH1; HHF4; M/SCHAD; SCHAD; Hydroxyacyl-coenzyme A dehydrogenase; mitochondrial Hydroxyacyl Coenzyme A dehydrogenase type II; Mitochondrial L3 Hydroxyacyl CoA Dehydrogenase; 17 beta hydroxysteroid dehydrogenase 10; 17 beta hydroxysteroid dehydrogenase type 10; 3 hydroxy 2 methylbutyryl CoA dehydrogenase; 3 hydroxyacyl CoA dehydrogenase type 2; 3 hydroxyacyl CoA dehydrogenase type II; AB binding alcohol dehydrogenase; 17b HSD10; ABAD; Ads9; Amyloid beta binding polypeptide; Amyloid beta peptide binding alcohol dehydrogenase; Amyloid beta peptide binding protein; CAMR; DuPXp11.22; Endoplasmic Reticulum Amyloid Binding Protein; Endoplasmic reticulum associated amyloid beta peptide binding protein; ER associated a+myloid beta-binding protein; ERAB; HADH 2; HADH2; HCD 2; HCD2; HSD17B10; Hydroxyacyl CoA Dehydrogenase type II; Hydroxysteroid (17 beta) dehydrogenase 10; Mental retardation X linked syndromic 11; MHBD; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; MRPP2; MRX17; Short chain dehydrogenase/reductase family 5C member 1; SDR5C1; Short chain L 3 hydroxyacyl CoA dehydrogenase type 2; Short chain type dehydrogenase/reductase XH98G2; Type 10 17b HSD; Type 10 17beta hydroxysteroid dehydrogenase; Type II HADH; XH98G2.

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 95% by SDS-PAGE

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

1.0 mg/ml (determined by Bradford assay) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of HADH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

HADH, which belongs to the family of oxidoreductases, is important for converting certain fats to energy. This protein is an enzyme that catalyzes the chemical reaction. ( (S)-3-hydroxyacyl-CoA + NAD+ <=>3-oxoacyl-CoA + NADH + H+) It is also involved in a process called fatty acid oxidation, in which several enzymes work in a step-wise fashion to break down (metabolize) fats and convert them to energy. Recombinant HADH protein was expressed in E Coli and purified by using conventional chromatography techniques.

Metabolism

SDS-PAGE

35.1 kDa (323aa), confirmed by MALDI-TOF.

hydroxyacyl-CoA dehydrogenase

hydroxyacyl-coenzyme A dehydrogenase, mitochondrial

Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial

HAD; HADHSC; SCHAD; HCDH  [Similar Products]

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq, May 2010]

Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.

Tieu K, et al. (2004) Ann Neurol. 56 (1):51-60; Vredendaal PJ, et al. (1996) Biochem Biophys Res Commun. 223 (3):718-23.;

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