ADH5, Polyclonal Antibody

Goat Polyclonal to Human ADH5; ADH5; Alcohol dehydrogenase 5; ADHX; Formaldehyde dehydrogenase; ADH-3; Alcohol dehydrogenase class-3; FALDH; GSH-FDH; GSNOR; FDH

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

This ADH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-239 amino acids from the Central region of human ADH5.

Protein A purified

PBS, 0.09% sodium azide

Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.

Small volumes of anti-ADH5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipidperoxidation products. The encoded protein forms a homodimer. It hasvirtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation ofS-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione.

Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

FC/FACS: 1:10-1:50
IHC-P: 1:10-1:100
WB: 1:1000

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot of ADH5 Antibody in K562 cell line lysates (35 ug/lane). ADH5 (arrow) was detected using the purified antibody.

Western blot of ADH5 (arrow) using rabbit polyclonal ADH5 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH5 gene.

Flow cytometric of K562 cells using ADH5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

39,724 Da

alcohol dehydrogenase 5 (class III), chi polypeptide

alcohol dehydrogenase class-3

Alcohol dehydrogenase class-3

FALDH; FDH; GSH-FDH  [Similar Products]

This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]

Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.