ADH5, Polyclonal Antibody

Alcohol dehydrogenase class-3; Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; ADH5

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

>95%, Protein G purified

Liquid

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-ADH5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

WB: 1: 500-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200

Western Blot
Positive WB detected in: Rat liver tissue
All lanes: ADH5 antibody at 2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40 KDa
Observed band size: 40 KDa

Immunohistochemistry of paraffin-embedded human liver cancer using MBS7047608 at dilution of 1:100

Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7047608 at dilution of 1:100

Immunofluorescent analysis of Hela cells using MBS7047608 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

39,724 Da

alcohol dehydrogenase 5 (class III), chi polypeptide

alcohol dehydrogenase class-3

Alcohol dehydrogenase class-3

FALDH; FDH; GSH-FDH  [Similar Products]

ADHX_HUMAN

This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]

ADH5: Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.

Protein type: Amino Acid Metabolism - tyrosine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Cofactor and Vitamin Metabolism - retinol; EC 1.1.1.1; EC 1.1.1.284; Energy Metabolism - methane; Lipid Metabolism - fatty acid; Mitochondrial; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol

Molecular Function: electron carrier activity; fatty acid binding; formaldehyde dehydrogenase activity; S-(hydroxymethyl)glutathione dehydrogenase activity; zinc ion binding

Biological Process: ethanol oxidation; response to redox state

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