ATR-interacting protein (ATRIP), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ATRIP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7213616 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ATR-interacting protein (ATRIP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATRIP. The ELISA analytical biochemical technique of the MBS7213616 kit is based on ATRIP antibody-ATRIP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATRIP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATRIP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

72,362 Da

ATR interacting protein

ATR-interacting protein; ATM and Rad3-related-interacting protein

ATR-interacting protein

AGS1  [Similar Products]

ATRIP_HUMAN

This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012]

ATRIP: is required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein. Heterodimerizes with ATR. The heterodimer binds replication protein A (RPA), a protein complex that subsequently is recruited to single stranded DNA (ssDNA). Its EEXXXDDL motif is required for the interaction with catalytic subunit of DNA-PK and its recruitment to sites of DNA damage. e for this protein is either identical to or adjacent to that of ATRIP. Some of the mRNAs that encode ATRIP also encode TREX1 in another reading frame. Three alternatively spliced human isoforms have been reported.

Protein type: DNA-binding; DNA repair, damage; Cell cycle regulation

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: DNA damage checkpoint; DNA repair; DNA replication

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