Product Name
NPHP1, siRNA
Full Product Name
NPHP1 siRNA (Mouse)
Product Synonym Names
NPH1; Nephrocystin-1
Product Gene Name
NPHP1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9QY53
Specificity
NPHP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NPHP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of NPHP1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NPHP1 sirna
siRNA to inhibit NPHP1 expression using RNA interference
Applications Tested/Suitable for NPHP1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for NPHP1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_058598.1
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NCBI GenBank Nucleotide #
NM_016902.4
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UniProt Primary Accession #
Q9QY53
[Other Products]
UniProt Secondary Accession #
Q9D7G8; Q9QZP0; Q9WUZ2[Other Products]
UniProt Related Accession #
Q9QY53[Other Products]
Molecular Weight
77,035 Da
NCBI Official Full Name
nephrocystin-1 isoform a
NCBI Official Synonym Full Names
nephronophthisis 1 (juvenile) homolog (human)
NCBI Official Symbol
Nphp1 [Similar Products]
NCBI Protein Information
nephrocystin-1
UniProt Protein Name
Nephrocystin-1
Protein Family
Nephrocystin
UniProt Gene Name
Nphp1 [Similar Products]
UniProt Synonym Gene Names
Nph1 [Similar Products]
UniProt Entry Name
NPHP1_MOUSE
UniProt Comments for NPHP1
NPHP1: Together with BCAR1 it may play a role in the control of epithelial cell polarity. Involved in the organization of apical junctions in kidney cells together with NPHP4 and RPGRIP1L/NPHP8. Does not seem to be strictly required for ciliogenesis. Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling. May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly. Required for normal retina development. In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19. Involved in spermatogenesis. Interacts with BCAR1, PTK2B/PYK2 and tensin. Interacts with INVS and NPHP3. Interacts with PACS1; the interaction is dependent on NPHP1 phosphorylation by CK2. Interatcs with KIF7. Interacts with AHI1 and TNK2. Interacts with NPHP4 in a complex containing NPHP1, NPHP4 and RPGRIP1L. Interacts with IQCB1; the interaction likely requires additional interactors. Widespread expression, with highest levels in pituitary gland, spinal cord, thyroid gland, testis, skeletal muscle, lymph node and trachea. Weakly expressed in heart, kidney and pancreas. Expressed in nasal epithelial cells. Belongs to the nephrocystin-1 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion
Cellular Component: cytoskeleton; tight junction; cell projection; cytoplasm; intercellular junction; cell junction; photoreceptor connecting cilium; cilium
Molecular Function: protein binding
Biological Process: spermatid differentiation; cell projection organization and biogenesis; retina development in camera-type eye; spermatogenesis; cell differentiation
Research Articles on NPHP1
1. PC-1 polyproline motif interacts with the SH3 domain of NPHP1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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