Product Name
PTDSS1, siRNA
Full Product Name
PTDSS1 siRNA (Human)
Product Synonym Names
KIAA0024; PSSA; Phosphatidylserine synthase 1; PSS-1; PtdSer synthase 1; Serine-exchange enzyme I
Product Gene Name
PTDSS1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P48651
Specificity
PTDSS1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PTDSS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PTDSS1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PTDSS1 sirna
siRNA to inhibit PTDSS1 expression using RNA interference
Applications Tested/Suitable for PTDSS1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PTDSS1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001277154.1
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NCBI GenBank Nucleotide #
NM_001290225.1
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UniProt Primary Accession #
P48651
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UniProt Secondary Accession #
Q9BUQ5; B4DE85; E5RFC5[Other Products]
UniProt Related Accession #
P48651[Other Products]
Molecular Weight
34,579 Da
NCBI Official Full Name
phosphatidylserine synthase 1 isoform 2
NCBI Official Synonym Full Names
phosphatidylserine synthase 1
NCBI Official Symbol
PTDSS1 [Similar Products]
NCBI Official Synonym Symbols
LMHD; PSS1; PSSA
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NCBI Protein Information
phosphatidylserine synthase 1
UniProt Protein Name
Phosphatidylserine synthase 1
UniProt Synonym Protein Names
Serine-exchange enzyme I
Protein Family
Phosphatidylserine synthase
UniProt Gene Name
PTDSS1 [Similar Products]
UniProt Synonym Gene Names
KIAA0024; PSSA; PSS-1; PtdSer synthase 1 [Similar Products]
UniProt Entry Name
PTSS1_HUMAN
NCBI Summary for PTDSS1
The protein encoded by this gene catalyzes the formation of phosphatidylserine from either phosphatidylcholine or phosphatidylethanolamine. Phosphatidylserine localizes to the mitochondria-associated membrane of the endoplasmic reticulum, where it serves a structural role as well as a signaling role. Defects in this gene are a cause of Lenz-Majewski hyperostotic dwarfism. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]
UniProt Comments for PTDSS1
PTDSS1: Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) or phosphatidylcholine (PC) is replaced by L-serine. In membranes, PTDSS1 catalyzes mainly the conversion of phosphatidylcholine. Also converts, in vitro and to a lesser extent, phosphatidylethanolamine. Belongs to the phosphatidyl serine synthase family.
Protein type: Membrane protein, multi-pass; EC 2.7.8.29; Membrane protein, integral; Transferase; Lipid Metabolism - glycerophospholipid; Mitochondrial
Chromosomal Location of Human Ortholog: 8q22
Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane
Molecular Function: transferase activity
Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylserine biosynthetic process
Disease: Lenz-majewski Hyperostotic Dwarfism
Research Articles on PTDSS1
1. Gain-of-function missense mutations in the phosphatidylserine synthase 1 (PTDSS1) gene cause Lenz-Majewski syndrome.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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