Probable arginyl-tRNA synthetase, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human RARS2. No significant cross-reactivity or interference between Human RARS2 and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of RARS2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS282473 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable arginyl-tRNA synthetase (RARS2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RARS2. The ELISA analytical biochemical technique of the MBS282473 kit is based on RARS2 antibody-RARS2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RARS2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RARS2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate RARS2 in samples. An antibody specific for RARS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRARS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RARS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RARS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

65,505 Da

arginyl-tRNA synthetase 2, mitochondrial

probable arginine--tRNA ligase, mitochondrial

Probable arginine--tRNA ligase, mitochondrial

RARSL; ArgRS  [Similar Products]

This nuclear gene encodes a protein that localizes to the mitochondria, where it catalyzes the transfer of L-arginine to its cognate tRNA, an important step in translation of mitochondrially-encoded proteins. Defects in this gene are a cause of pontocerebellar hypoplasia type 6 (PCH6). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

RARS2: Defects in RARS2 are the cause of pontocerebellar hypoplasia type 6 (PCH6); also known as fatal infantile encephalopathy with mitochondrial respiratory chain defects. Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem. Belongs to the class-I aminoacyl-tRNA synthetase family.

Protein type: Aminoacyl-tRNA synthetase; EC 6.1.1.19; Ligase; Mitochondrial; Translation; Translation regulation

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: arginine-tRNA ligase activity; ATP binding; RNA binding

Biological Process: arginyl-tRNA aminoacylation; mitochondrial translation; tRNA aminoacylation for protein translation

Disease: Pontocerebellar Hypoplasia, Type 6

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