Full Product Name
A2M Monoclonal Antibody
Product Synonym Names
A2m; A2MG; Alpha 2 M; Alpha 2M; Alpha-2-M; Alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; DKFZp779B086; FWP007; S863 7
Product Gene Name
anti-A2M antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
103950 geneneXtProt; the human protein knowledge platformMore
3D Structure
ModBase 3D Structure for P01023
Purity/Purification
Protein A purification
Concentration
1mg/mL (lot specific)
Immunogen
Fusion protein of Alpha-2-macroglobulin
Buffer
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-A2M antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-A2M antibody
alpha-2-macroglobulin, also known as alpha2-macroglobulin (alpha2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.
Product Categories/Family for anti-A2M antibody
Cancer; Cell Biology; Cardiovascular
Applications Tested/Suitable for anti-A2M antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-A2M antibody
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
Western Blot (WB) of anti-A2M antibody
Western Blot analysis of HepG2 cells using Alpha-2-macroglobulin Monoclonal Antibody at dilution of 1:1000
NCBI/Uniprot data below describe general gene information for A2M. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000005.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000014.5
[Other Products]
UniProt Primary Accession #
P01023
[Other Products]
UniProt Secondary Accession #
Q13677; Q59F47; Q5QTS0; Q68DN2; Q6PIY3; Q6PN97[Other Products]
UniProt Related Accession #
P01023[Other Products]
Molecular Weight
Calculated: 163kDa
Observed: 185kDa
NCBI Official Full Name
alpha-2-macroglobulin isoform a
NCBI Official Synonym Full Names
alpha-2-macroglobulin
NCBI Official Symbol
A2M [Similar Products]
NCBI Official Synonym Symbols
A2MD; CPAMD5; FWP007; S863-7
[Similar Products]
NCBI Protein Information
alpha-2-macroglobulin
UniProt Protein Name
Alpha-2-macroglobulin
UniProt Synonym Protein Names
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Protein Family
Alpha-2-macroglobulin
UniProt Gene Name
A2M [Similar Products]
UniProt Synonym Gene Names
CPAMD5; Alpha-2-M [Similar Products]
NCBI Summary for A2M
The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified. [provided by RefSeq, Nov 2016]
UniProt Comments for A2M
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Research Articles on A2M
1. The blood coagulation pathway may be a key regulatory network pathway contributing to the allergic inflammatory response in AR patients. A2M, which is regulated by STAT3, may be an important protein in the pathogenesis of allergic rhinitis in AR patients.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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