Glucokinase, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 11; NC_000077.6 (5900823..5949599, complement). Location: 11 A1; 11 3.88 cM

Store all reagents at 2-8 degree C

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of GCK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS043825 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glucokinase (GCK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GCK. The ELISA analytical biochemical technique of the MBS043825 kit is based on GCK antibody-GCK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GCK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GCK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

52,089 Da

glucokinase

glucokinase; HK IV; hexokinase 4; hexokinase-4; hexokinase-D; hexokinase type IV

Glucokinase

Gk; HK IV; HK4  [Similar Products]

HXK4_MOUSE

GK: a glycolytic enzyme that catalyzes glucose metabolism in the liver and pancreatic beta cells. Acts as a ""glucose sensor"" in beta cells. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. Glucokinase traps glucose inside the cell by catalyzing its phosphorylation to produce glucose-6-phosphate. Has a lower affinity for glucose than the three other isozymes of hexokinase, allowing other organs such as the brain and muscles to have first call on glucose when its supply is limited. Unlike other hexokinases, glucokinase is not inhibited by glucose-6-phosphate. Glucokinase is found in the outer membrane compartment of mitochondria. May bind VDAC, suppressing mitochondrial function. Glucokinase transcription is induced by insulin, perhaps via the activation of Stat 5B. Mutant glucokinase causes a rare form of diabetes and may also play a role in type 2 diabetes. Three splice variant isoforms of human glucokinase have been described.

Protein type: Carbohydrate Metabolism - starch and sucrose; Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Mitochondrial; Carbohydrate Metabolism - galactose; EC 2.7.1.2; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Cellular Component: nucleoplasm; mitochondrion; cell; cytoplasm; actin filament; cell cortex; basal cortex; nucleus; cytosol; secretory granule

Molecular Function: transferase activity; protein binding; hexokinase activity; magnesium ion binding; nucleotide binding; kinase activity; ADP binding; phosphotransferase activity, alcohol group as acceptor; glucokinase activity; protein phosphatase binding; ATP binding; glucose binding

Biological Process: second-messenger-mediated signaling; glycogen biosynthetic process; glycolysis; regulation of potassium ion transport; positive regulation of glycogen biosynthetic process; glucose 6-phosphate metabolic process; lipid homeostasis; positive regulation of insulin secretion; glucose metabolic process; glucose homeostasis; NADP metabolic process; fructose 2,6-bisphosphate metabolic process; cellular response to glucose starvation; elevation of cytosolic calcium ion concentration; detection of glucose; cellular response to insulin stimulus; negative regulation of gluconeogenesis; carbohydrate metabolic process; carbohydrate phosphorylation; positive regulation of glycolysis; negative regulation of epinephrine secretion; cell glucose homeostasis; regulation of insulin secretion; phosphorylation; positive regulation of phosphorylation

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