TGM5, Polyclonal Antibody

TGX; PSS2; TGM6; TGMX; TGASE5; TGASEX

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total TGM5 protein.

Antigen Affinity Purification

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

1 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-TGM5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the transglutaminase family. The encoded protein catalyzes formation of protein cross-links between glutamine and lysine residues, often resulting in stabilization of protein assemblies. This reaction is calcium dependent. Mutations in this gene have been associated with acral peeling skin syndrome.

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC)

Western Blot: 1:500-1:2000
Immunohistochemistry: 1:25-1:100

Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-5: Hela cells, A431 cells, Mouse skin tissue, MCF7 cells, K562 cells, Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20.

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue using at dilution 1/20.

71,919 Da

transglutaminase 5

protein-glutamine gamma-glutamyltransferase 5

Protein-glutamine gamma-glutamyltransferase 5

TGMX; TG(X); TGX; TGase X; TGase-5  [Similar Products]

TGM5_HUMAN

TGM5: Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Contributes to the formation of the cornified cell envelope of keratinocytes. Defects in TGM5 are a cause of peeling skin syndrome type A (APSS). A non-inflammatory form of peeling skin syndrome, a genodermatosis characterized by the continuous shedding of the outer layers of the epidermis. In APSS patients, skin peeling is strictly limited to the dorsa of the hands and feet, and it is accompanied by accompanied by painless erythema and spontaneous non-scarring healing. Ultrastructural and histological analysis shows a level of blistering high in the epidermis at the stratum granulosum-stratum corneum junction. Belongs to the transglutaminase superfamily. Transglutaminase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.2.13; Transferase

Chromosomal Location of Human Ortholog: 15q15.2

Biological Process: epidermis development; protein modification process

Disease: Peeling Skin Syndrome 2

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