40S ribosomal protein S3 (RPS3), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of RPS3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7216596 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 40S ribosomal protein S3 (RPS3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RPS3. The ELISA analytical biochemical technique of the MBS7216596 kit is based on RPS3 antibody-RPS3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RPS3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RPS3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

28,487 Da

ribosomal protein S3

40S ribosomal protein S3; IMR-90 ribosomal protein S3

40S ribosomal protein S3

OK/SW-cl.26  [Similar Products]

RS3_HUMAN

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

RPS3: a component of the 40S ribosomal subunit of eukaryotes. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Overexpressed in colorectal cancer.

Protein type: Translation; Ribosomal; Apoptosis; EC 4.2.99.18; RNA-binding

Chromosomal Location of Human Ortholog: 11q13.3-q13.5

Cellular Component: focal adhesion; membrane; cytoplasm; mitochondrial inner membrane; nucleolus; spindle; ribonucleoprotein complex; nucleus; cytosol

Molecular Function: mRNA binding; NF-kappaB binding; protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; enzyme binding; structural constituent of ribosome; protein kinase A binding; oxidized purine base lesion DNA N-glycosylase activity; iron-sulfur cluster binding; damaged DNA binding; protein kinase binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; mitosis; viral reproduction; translation; transcription, DNA-dependent; apoptosis; translational termination; viral infectious cycle; DNA repair; DNA catabolic process, endonucleolytic; activation of NF-kappaB transcription factor; regulation of translation; translational elongation; cellular protein metabolic process; cell division; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; response to DNA damage stimulus; negative regulation of DNA repair

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