Product Name
Non-enzymatic Human tPA (PLAT), Protein
Full Product Name
Non-enzymatic Human tPA
Product Gene Name
PLAT protein
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 8; NC_000008.10 (42032236..42065194, complement). Location: 8p12
3D Structure
ModBase 3D Structure for P00750
Purity/Purification
>95% by SDS-PAGE analysis
Form/Format
Frozen liquid
Concentration
2.0 mg/ml (lot specific)
Buffer
0.4M HEPES; 0.1M NaCl; pH 7.4
Extinction Coefficient
1.9
Preparation and Storage
Store at -70 degree C. Shelf Life: 3 years from delivery
Other Notes
Small volumes of PLAT protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PLAT protein
This human tPA has an active site serine to alanine mutation which renders it catalytically inactive. The mutation site is S478A on the mature protein and S513A on the complete mRNA sequence (UniProtKB: locus TPA_HUMAN, accession P00750). It is >90percent
Product Categories/Family for PLAT protein
Tissue Plasminogen Activator (tPA); Human tPA
NCBI/Uniprot data below describe general gene information for PLAT. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000921.1
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NCBI GenBank Nucleotide #
NM_000930.3
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UniProt Primary Accession #
P00750
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UniProt Secondary Accession #
Q15103; Q503B0; Q6PJA5; Q7Z7N2; Q86YK8; Q9BU99; Q9BZW1; A8K022; B2R8E8[Other Products]
UniProt Related Accession #
P00750[Other Products]
NCBI Official Full Name
tissue-type plasminogen activator isoform 1 preproprotein
NCBI Official Synonym Full Names
plasminogen activator, tissue
NCBI Official Symbol
PLAT [Similar Products]
NCBI Official Synonym Symbols
TPA; T-PA
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NCBI Protein Information
tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA)
UniProt Protein Name
Tissue-type plasminogen activator
Protein Family
Tissue-type plasminogen activator
UniProt Gene Name
PLAT [Similar Products]
UniProt Synonym Gene Names
t-PA; t-plasminogen activator; tPA [Similar Products]
UniProt Entry Name
TPA_HUMAN
NCBI Summary for PLAT
This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Comments for PLAT
PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Motility/polarity/chemotaxis; Protease; Vesicle; EC 3.4.21.68; Cytoskeletal; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8p12
Cellular Component: extracellular matrix; extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule
Molecular Function: protein binding; serine-type endopeptidase activity
Biological Process: negative regulation of proteolysis; synaptic transmission, glutamatergic; response to peptide hormone stimulus; response to cAMP; smooth muscle cell migration; regulation of synaptic plasticity; protein modification process; platelet-derived growth factor receptor signaling pathway; proteolysis; fibrinolysis; response to hypoxia; blood coagulation; transmembrane transport; plasminogen activation
Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator
Research Articles on PLAT
1. These results suggest that retained tPA on vascular endothelial cells effectively activated plasminogen to plasmin.
Precautions
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