Non-enzymatic Mouse tPA, Protein

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 8; NC_000074.6 (22757744..22782848). Location: 8 A2; 8 11.42 cM

Insect cell culture

>95% by SDS-PAGE analysis

Frozen liquid

2.0 mg/ml (lot specific)

Store at -70 degree C. Shelf Life: 3 years from delivery

Small volumes of PLAT protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This single chain mouse tPA has the active site serine mutated to alanine rendering the enzyme catalytically inactive. The mutation site is S481A on the mature protein and S510A on the complete mRNA sequence (UniProtKB: locus TPA_MOUSE, accession P11214).

Tissue Plasminogen Activator (tPA); Mouse tPA

70,000

plasminogen activator, tissue

tissue-type plasminogen activator; t-plasminogen activator

Tissue-type plasminogen activator

t-PA; t-plasminogen activator; tPA  [Similar Products]

TPA_MOUSE

This gene encodes a key enzyme of the fibrinolytic pathway. The encoded protein undergoes proteolytic processing by plasmin to generate a heterodimeric serine protease that cleaves the proenzyme plasminogen to produce plasmin, a protease that is required to break down fibrin clots. Additionally, the encoded protein is involved in other biological processes such as synaptic plasticity, cell migration and tissue remodeling. Mice lacking the encoded protein display a reduction in long-term potentiation in hippocampus and conversely, transgenic mice overexpressing the encoded protein have increased and prolonged long-term potentiation. [provided by RefSeq, Jul 2015]

PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Protease; Secreted, signal peptide; Secreted; EC 3.4.21.68; Vesicle

Cellular Component: extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: negative regulation of proteolysis; synaptic transmission, glutamatergic; smooth muscle cell migration; response to hypoxia; platelet-derived growth factor receptor signaling pathway; proteolysis; plasminogen activation

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