ACCalpha, Polyclonal Antibody

ACCa (Phospho-Ser80) Polyclonal Antibody; ACACA; ACAC; ACC1; ACCA; Acetyl-CoA carboxylase 1; ACC1; ACC-alpha

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Phospho-ACCa (S80) Polyclonal Antibody detects endogenous levels of ACCa protein only when phosphorylated at S80.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

1 mg/ml (lot specific)

Store at -20 degree C/1 year

Small volumes of anti-ACCalpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Phospho-specific Ab

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:100 - 1:300
ELISA: 1:5000
Not yet tested in other applications

Western Blot analysis of HELA cells using Phospho-ACCa (S80) Polyclonal Antibody

269,999 Da

acetyl-CoA carboxylase alpha

acetyl-CoA carboxylase 1

Acetyl-CoA carboxylase 1

ACAC; ACC1; ACCA; ACC1  [Similar Products]

ACACA_HUMAN

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

ACC1: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC-alpha is the predominant isoform in liver, adipocyte and mammary gland. ACC-beta is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity.

Protein type: Lipid Metabolism - fatty acid biosynthesis; Ligase; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - propanoate; EC 6.4.1.2; EC 6.3.4.14

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: mitochondrion; cytoplasm; nucleolus; cytosol; actin cytoskeleton

Molecular Function: protein binding; acetyl-CoA carboxylase activity; metal ion binding; biotin carboxylase activity; ATP binding

Biological Process: vitamin metabolic process; acetyl-CoA metabolic process; multicellular organismal protein metabolic process; tissue homeostasis; energy reserve metabolic process; positive regulation of cellular metabolic process; lipid homeostasis; triacylglycerol biosynthetic process; cellular lipid metabolic process; carnitine shuttle; biotin metabolic process; water-soluble vitamin metabolic process; fatty acid biosynthetic process; protein homotetramerization

Disease: Acetyl-coa Carboxylase Deficiency

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