Full Product Name
GHR siRNA (Human)
Product Synonym Names
Growth hormone receptor; GH receptor; Somatotropin receptor
Product Gene Name
GHR sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P10912
Specificity
GHR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GHR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GHR sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GHR sirna
siRNA to inhibit GHR expression using RNA interference
Applications Tested/Suitable for GHR sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GHR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000154.1
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NCBI GenBank Nucleotide #
NM_000163.4
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UniProt Primary Accession #
P10912
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UniProt Secondary Accession #
Q9HCX2[Other Products]
UniProt Related Accession #
P10912[Other Products]
Molecular Weight
69,237 Da
NCBI Official Full Name
growth hormone receptor isoform 1
NCBI Official Synonym Full Names
growth hormone receptor
NCBI Official Symbol
GHR [Similar Products]
NCBI Official Synonym Symbols
GHBP; GHIP
[Similar Products]
NCBI Protein Information
growth hormone receptor
UniProt Protein Name
Growth hormone receptor
UniProt Synonym Protein Names
Somatotropin receptor
UniProt Gene Name
GHR [Similar Products]
UniProt Synonym Gene Names
GH receptor; GH-binding protein; GHBP [Similar Products]
UniProt Entry Name
GHR_HUMAN
NCBI Summary for GHR
This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]
UniProt Comments for GHR
GH receptor: Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. Defects in GHR are a cause of Laron syndrome (LARS). A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may be a cause of idiopathic short stature autosomal (ISSA). Short stature is defined by a subnormal rate of growth. Belongs to the type I cytokine receptor family. Type 1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, cytokine; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5p13-p12
Cellular Component: extracellular space; cell surface; integral to plasma membrane; extracellular region; integral to membrane; plasma membrane; receptor complex
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protein homodimerization activity; peptide hormone binding; growth factor binding; protein kinase binding
Biological Process: succinate metabolic process; oxaloacetate metabolic process; activation of MAPK activity; positive regulation of multicellular organism growth; fatty acid metabolic process; tyrosine phosphorylation of JAK2 protein; response to estradiol stimulus; 2-oxoglutarate metabolic process; positive regulation of tyrosine phosphorylation of Stat3 protein; allantoin metabolic process; receptor internalization; creatinine metabolic process; isoleucine metabolic process; cytokine and chemokine mediated signaling pathway; regulation of multicellular organism growth; citrate metabolic process; valine metabolic process; endocytosis; JAK-STAT cascade; creatine metabolic process; multicellular organismal metabolic process; cellular response to hormone stimulus; positive regulation of peptidyl-tyrosine phosphorylation; insulin-like growth factor receptor signaling pathway; positive regulation of tyrosine phosphorylation of Stat5 protein; response to cycloheximide; taurine metabolic process
Disease: Hypercholesterolemia, Familial; Laron Syndrome
Research Articles on GHR
1. The E180splice mutation in the GHR gene causing Laron syndrome.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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