Glypican 4, aa54-66, Polyclonal Antibody

Anti -Glypican 4, aa54-66 (K-glypican, UNQ474/PRO937, GPC4)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: X; NC_000023.10 (132435064..132549205, complement). Location: Xq26.1

Polyclonal

Rabbit

Specific for a core protein epitope (aa 54-66) of human glypican-4.

Serum
Serum

Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in ddH2O.

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-GPC4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Glypican-4, previously known as K-glypican, is a GPI-linked cell surface heparan sulfate proteoglycan of the glypican gene family, that comprises 6 members at present. Its function is only partially understood at present. However, glypican-4 seems to be involved in the control of cell growth and diffe-rentiation, especially in the development of the central nervous system and the kidney. Furthermore, glypican-4 is expressed by haematopoetic stem cells and bone marrow stromal cells. Although the antiserum was prepared against a peptide from hu man glypican-4, due to the highly conserved structure and se-quence of glypican-4, antiserum anti-peptide 1 (aa88-101) was used successfully to study brain development in the mouse.

Antibodies; Abs to Proteoglycans

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)

Suitable for use in ELISA, Western Blotting, Immunohistochemistry.
Dilution: Western Blotting: 1:1000-1:2000
Immunohistochemistry: 1:1000

62,412 Da[Similar Products]

glypican 4

glypican-4; OTTHUMP00000024052; dJ900E8.1 (glypican 4); glypican proteoglycan 4

Glypican-4

GPC4_HUMAN

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. [provided by RefSeq]

GPC4: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system. Belongs to the glypican family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: proteinaceous extracellular matrix; lysosomal lumen; extracellular space; Golgi lumen; integral to plasma membrane; plasma membrane; nucleus; external side of plasma membrane

Molecular Function: heparan sulfate proteoglycan binding

Biological Process: chondroitin sulfate metabolic process; phototransduction, visible light; cell proliferation; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; anatomical structure morphogenesis; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; retinoid metabolic process

Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1

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