Product Name
HYDIN, siRNA
Full Product Name
HYDIN siRNA (Human)
Product Synonym Names
HYDIN1; KIAA1864; Hydrocephalus-inducing protein homolog
Product Gene Name
HYDIN sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q4G0P3
Specificity
HYDIN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HYDIN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of HYDIN sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HYDIN sirna
siRNA to inhibit HYDIN expression using RNA interference
Applications Tested/Suitable for HYDIN sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for HYDIN. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001185471.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001198542.1
[Other Products]
UniProt Primary Accession #
Q4G0P3
[Other Products]
UniProt Secondary Accession #
Q8N3H8; Q8N3P6; Q8TC08; Q96JG3; Q96SS4; Q9H5U3; A6NC70; A6NLZ0; B4DQY4; B4DRN4; F5H6V3[Other Products]
UniProt Related Accession #
Q4G0P3[Other Products]
Molecular Weight
105,338 Da
NCBI Official Full Name
hydrocephalus-inducing protein homolog isoform c
NCBI Official Synonym Full Names
HYDIN, axonemal central pair apparatus protein
NCBI Official Symbol
HYDIN [Similar Products]
NCBI Official Synonym Symbols
CILD5; HYDIN1; HYDIN2; PPP1R31
[Similar Products]
NCBI Protein Information
hydrocephalus-inducing protein homolog
UniProt Protein Name
Hydrocephalus-inducing protein homolog
Protein Family
Hydrocephalus-inducing protein
UniProt Gene Name
HYDIN [Similar Products]
UniProt Synonym Gene Names
HYDIN1; KIAA1864 [Similar Products]
UniProt Entry Name
HYDIN_HUMAN
NCBI Summary for HYDIN
This gene encodes a protein that may be involved in cilia motility. Mutations in this gene cause of autosomal recessive primary ciliary dyskinesia-5, a disorder characterized by the accumulation of cerebrospinal fluid within the ventricles of the brain. A duplicate copy of this gene has been found in humans on chromosome 1. [provided by RefSeq, Jan 2013]
UniProt Comments for HYDIN
HYDIN: 5 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 16q22.2
Cellular Component: cilium
Biological Process: ventricular system development; cilium biogenesis; epithelial cell development
Disease: Ciliary Dyskinesia, Primary, 5
Research Articles on HYDIN
1. The results suggest frequent and coordinated adaptive immune responses against HYDIN variants in patients with cancer.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
