Full Product Name
c-Kit (Ab-721) Antibody
Product Gene Name
anti-c-Kit antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P10721
Species Reactivity
Human, Mouse, Rat
Specificity
c-Kit (Ab-721) Antibody detects endogenous levels of total c-Kit protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human c-Kit around the phosphorylation site of tyrosine 721 (N-E-YP-M-D)
Preparation and Storage
Store at-20 degree C for 1 year
Other Notes
Small volumes of anti-c-Kit antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-c-Kit antibody
Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
Applications Tested/Suitable for anti-c-Kit antibody
Western Blot (WB)
Application Notes for anti-c-Kit antibody
WB: 1:500-1000
Western Blot (WB) of anti-c-Kit antibody
Western blot analysis of extract from A431 cell treated with EGF (200ng/ml, 30min), using c-Kit (Ab-721) Antibody.
NCBI/Uniprot data below describe general gene information for c-Kit. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000213.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000222.2
[Other Products]
UniProt Primary Accession #
P10721
[Other Products]
UniProt Secondary Accession #
Q6IQ28; Q99662; Q9UM99; B5A956; D5LXN2; D5M931; F5H8F8[Other Products]
UniProt Related Accession #
P10721[Other Products]
Molecular Weight
46,658 Da
NCBI Official Full Name
mast/stem cell growth factor receptor Kit isoform 1
NCBI Official Synonym Full Names
KIT proto-oncogene receptor tyrosine kinase
NCBI Official Symbol
KIT [Similar Products]
NCBI Official Synonym Symbols
PBT; SCFR; C-Kit; CD117
[Similar Products]
NCBI Protein Information
mast/stem cell growth factor receptor Kit
UniProt Protein Name
Mast/stem cell growth factor receptor Kit
UniProt Synonym Protein Names
Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD_antigen: CD117
UniProt Gene Name
KIT [Similar Products]
UniProt Synonym Gene Names
SCFR; SCFR; PBT [Similar Products]
NCBI Summary for c-Kit
This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for c-Kit
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
Product References and Citations for anti-c-Kit antibody
Kimura W, et al.(2007)Hepatogastroenterology.54(80):2203-8. Naeem M, et al.(2002)Hum Pathol. 33(12):1182-7. Jankulovski N, et al.(2006)Prilozi. 27(2):59-70.
Research Articles on c-Kit
1. CD117 can be a useful marker to help differentiate plasmablastic plasma cell myeloma from plasmablastic lymphoma
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
