c-Kit, Polyclonal Antibody

c-Kit Rabbit pab Antibody

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 4; NC_000004.11 (55524095..55606881). Location: 4q11-q12

Polyclonal

IgG

Rabbit

Affinity purified

Liquid

1mg/ml (lot specific)

Upon delivery aliquot and store at -20 degree C or -80 degree C

Small volumes of anti-c-Kit antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCgamma, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).

Western Blot (WB), Immunohistochemistry (IHC)

WB: 1/500-1000; IHC: 1/200

Western blot analysis of extracts of various cell lines, using KIT antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

109,865 Da

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

mast/stem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Mast/stem cell growth factor receptor Kit

SCFR; SCFR; PBT  [Similar Products]

KIT_HUMAN

1. Martin, F.H. et al. (1990) Cell 63, 203-11.
2. Yarden, Y. et al. (1987) EMBO J 6, 3341-51.
3. Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
4. Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
5. Nocka, K. et al. (1990) EMBO J 9, 1805-13.

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