Phosphodiesterase 8B (PDE8B), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 5; NC_000005.9 (76506706..76724081). Location: 5q13.3

Store all reagents at 2-8 degree C

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Small volumes of PDE8B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9313112 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphodiesterase 8B (PDE8B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDE8B. The ELISA analytical biochemical technique of the MBS9313112 kit is based on PDE8B antibody-PDE8B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDE8B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDE8B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

98,979 Da

phosphodiesterase 8B

high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B; hsPDE8B; cell proliferation-inducing gene 22 protein; 3',5' cyclic nucleotide phosphodiesterase 8B

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B

HsPDE8B  [Similar Products]

PDE8B_HUMAN

The protein encoded by this gene is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. The encoded protein, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in this gene are a cause of autosomal dominant striatal degeneration (ADSD). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]

PDE8B: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland. Defects in PDE8B are the cause of striatal degeneration autosomal dominant (ADSD). It is a movement disorder affecting the striatal part of the basal ganglia and characterized by bradykinesia, dysarthria and muscle rigidity. These symptoms resemble idiopathic Parkinson disease, but tremor is not present. Defects in PDE8B are the cause of primary pigmented nodular adrenocortical disease type 3 (PPNAD3). PPNAD3 is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the adrenals is characteristic with small pigmented micronodules observed in the cortex. Adrenal glands show overall normal size and weight, and multiple small yellow-to-dark brown nodules surrounded by a cortex with a uniform appearance. Microscopically, there are moderate diffuse cortical hyperplasia with mostly nonpigmented nodules, multiple capsular deficits and massive circumscribed and infiltrating extra-adrenal cortical excrescences with micronodules. Clinical manifestations of Cushing syndrome include facial and trunkal obesity, abdominal striae, muscular weakness, osteoporosis, arterial hypertension, diabetes. Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphodiesterase; Nucleotide Metabolism - purine; EC 3.1.4.53

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; signal transducer activity; metal ion binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: cyclic nucleotide metabolic process; behavioral fear response; operant conditioning; cAMP catabolic process; two-component signal transduction system (phosphorelay); visual learning; neuromuscular process controlling balance; negative regulation of insulin secretion

Disease: Striatal Degeneration, Autosomal Dominant; Pigmented Nodular Adrenocortical Disease, Primary, 3

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