myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila), E

Mouse Histone-lysine N-methyltransferase MLL5 (MLL5) ELISA kit; FLJ10078; FLJ14026; HDCMC04P; KMT2E; MGC70452; myeloid/lymphoid or mixed-lineage leukemia 5; myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog; Drosophila)

For Research Use Only. Not for use in diagnostic procedures.

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MLL5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9330243 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila) (MLL5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MLL5. The ELISA analytical biochemical technique of the MBS9330243 kit is based on MLL5 antibody-MLL5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MLL5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MLL5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of MLL5 (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

204,543 Da

lysine (K)-specific methyltransferase 2E

histone-lysine N-methyltransferase 2E; histone-lysine N-methyltransferase MLL5; myeloid/lymphoid or mixed-lineage leukemia 5; myeloid/lymphoid or mixed-lineage leukemia protein 5 homolog

Histone-lysine N-methyltransferase 2E

Mll5; Lysine N-methyltransferase 2E  [Similar Products]

KMT2E_MOUSE

MLL5: Histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys- 4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic- acid-induced granulopoiesis by acting as a coactivator of RAR- alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases. Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.43; Methyltransferase, protein lysine; Methyltransferase; Cell cycle regulation; Transcription, coactivator/corepressor

Cellular Component: nucleoplasm; intercellular bridge; intracellular membrane-bound organelle; nucleus

Molecular Function: transferase activity; methyltransferase activity; enzyme binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); metal ion binding; transcription coactivator activity; histone-lysine N-methyltransferase activity

Biological Process: methylation; retinoic acid receptor signaling pathway; neutrophil activation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone H3-K4 methylation; positive regulation of granulocyte differentiation; neutrophil mediated immunity; chromatin modification; cell cycle; peptidyl-lysine methylation; regulation of transcription, DNA-dependent; DNA methylation; erythrocyte differentiation; cell cycle arrest

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