myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila), E

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

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Small volumes of KMT2E elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2602819 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila) (KMT2E) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KMT2E. The ELISA analytical biochemical technique of the MBS2602819 kit is based on KMT2E antibody-KMT2E antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KMT2E antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KMT2E. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

131,737 Da[Similar Products]

lysine (K)-specific methyltransferase 2E

histone-lysine N-methyltransferase 2E; histone-lysine N-methyltransferase MLL5; myeloid/lymphoid or mixed-lineage leukemia protein 5; myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila)

Histone-lysine N-methyltransferase 2E

MLL5; Lysine N-methyltransferase 2E  [Similar Products]

KMT2E_HUMAN

This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a protein with an N-terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

MLL5: Histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys- 4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic- acid-induced granulopoiesis by acting as a coactivator of RAR- alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases. Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; Methyltransferase, protein lysine; EC 2.1.1.43; Transcription, coactivator/corepressor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleoplasm; intercellular bridge; intracellular membrane-bound organelle; cytoplasm; plasma membrane; nuclear speck

Molecular Function: protein binding; enzyme binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); transcription coactivator activity

Biological Process: retinoic acid receptor signaling pathway; establishment and/or maintenance of chromatin architecture; neutrophil activation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone H3-K4 methylation; positive regulation of granulocyte differentiation; neutrophil mediated immunity; DNA methylation; erythrocyte differentiation; cell cycle arrest

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