Product Name
ARL13B, siRNA
Full Product Name
ARL13B siRNA (Mouse)
Product Synonym Names
ARL2L1; ADP-ribosylation factor-like protein 13B; ADP-ribosylation factor-like protein 2-like 1; ARL2-like protein 1; Protein hennin
Product Gene Name
ARL13B sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q640N2
Specificity
ARL13B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ARL13B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ARL13B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ARL13B sirna
siRNA to inhibit ARL13B expression using RNA interference
Applications Tested/Suitable for ARL13B sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ARL13B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_080853.3
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NCBI GenBank Nucleotide #
NM_026577.3
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UniProt Primary Accession #
Q640N2
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UniProt Secondary Accession #
G3X9K5[Other Products]
UniProt Related Accession #
Q640N2[Other Products]
Molecular Weight
48,144 Da
NCBI Official Full Name
ADP-ribosylation factor-like protein 13B
NCBI Official Synonym Full Names
ADP-ribosylation factor-like 13B
NCBI Official Symbol
Arl13b [Similar Products]
NCBI Official Synonym Symbols
hnn; Arl2l1; A530097K21Rik; A930014M17Rik; C530009C10Rik
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NCBI Protein Information
ADP-ribosylation factor-like protein 13B
UniProt Protein Name
ADP-ribosylation factor-like protein 13B
UniProt Synonym Protein Names
ADP-ribosylation factor-like protein 2-like 1; ARL2-like protein 1; Protein hennin
UniProt Gene Name
Arl13b [Similar Products]
UniProt Synonym Gene Names
Arl2l1; ARL2-like protein 1 [Similar Products]
UniProt Entry Name
AR13B_MOUSE
UniProt Comments for ARL13B
ARL13B: Defects in ARL13B are the cause of Joubert syndrome type 8 (JBTS8). JBTS is an autosomal recessive disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermis hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. Belongs to the small GTPase superfamily. Arf family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: G protein, monomeric, ARF; G protein, monomeric
Cellular Component: cell projection; membrane; plasma membrane; intracellular; cilium
Molecular Function: GTP binding; nucleotide binding
Biological Process: formation of radial glial scaffolds; smoothened signaling pathway; dorsal/ventral pattern formation; interneuron migration from the subpallium to the cortex; sensory cilium biogenesis; small GTPase mediated signal transduction; multicellular organismal development; neural tube patterning; cilium biogenesis; heart looping; determination of left/right symmetry
Research Articles on ARL13B
1. A new role for Arl13b in actin cytoskeleton remodeling through the interaction with Myh9.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
