C/EBPepsilon, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This kit recognizes natural and recombinant Chicken C/EBPepsilon. No significant cross-reactivity or interference between Chicken C/EBPepsilon and analogues was observed.

Store at 4 degree C.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of C/EBPepsilon elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2504677 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C/EBPepsilon, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C/EBPepsilon. The ELISA analytical biochemical technique of the MBS2504677 kit is based on C/EBPepsilon antibody-C/EBPepsilon antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C/EBPepsilon antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C/EBPepsilon. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken C/EBPepsilon concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C/EBPepsilon. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for C/EBPepsilon and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C/EBPepsilon, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of C/EBPepsilon. You can calculate the concentration of C/EBPepsilon in the samples by comparing the OD of the samples to the standard curve.

30,603 Da

CCAAT/enhancer binding protein (C/EBP), epsilon

CCAAT/enhancer-binding protein epsilon; c/EBP epsilon

CCAAT/enhancer-binding protein epsilon

C/EBP epsilon  [Similar Products]

CEBPE_HUMAN

The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

C/EBP-epsilon: C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Belongs to the bZIP family. C/EBP subfamily.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm

Molecular Function: protein homodimerization activity; protein heterodimerization activity

Biological Process: macrophage differentiation; transcription, DNA-dependent; cytokine biosynthetic process; defense response to bacterium; defense response; positive regulation of transcription from RNA polymerase II promoter; phagocytosis

Disease: Specific Granule Deficiency

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