Full Product Name
CNBP siRNA (Rat)
Product Synonym Names
ZNF9; Cellular nucleic acid-binding protein; CNBP; Zinc finger protein 9
Product Gene Name
CNBP sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P62634
Specificity
CNBP siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CNBP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CNBP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CNBP sirna
siRNA to inhibit CNBP expression using RNA interference
Applications Tested/Suitable for CNBP sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CNBP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_072120.1
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NCBI GenBank Nucleotide #
NM_022598.1
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UniProt Primary Accession #
P62634
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UniProt Secondary Accession #
P20694; Q5QJQ8[Other Products]
UniProt Related Accession #
P62634[Other Products]
Molecular Weight
19,463 Da
NCBI Official Full Name
cellular nucleic acid-binding protein
NCBI Official Synonym Full Names
CCHC-type zinc finger, nucleic acid binding protein
NCBI Official Symbol
Cnbp [Similar Products]
NCBI Official Synonym Symbols
Znf9; Cnbp1
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NCBI Protein Information
cellular nucleic acid-binding protein
UniProt Protein Name
Cellular nucleic acid-binding protein
UniProt Synonym Protein Names
Zinc finger protein 9
Protein Family
Cellular nucleic acid-binding protein
UniProt Gene Name
Cnbp [Similar Products]
UniProt Synonym Gene Names
Znf9; CNBP [Similar Products]
UniProt Entry Name
CNBP_RAT
NCBI Summary for CNBP
nucleic acid binding protein [RGD, Feb 2006]
UniProt Comments for CNBP
ZNF9: Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression. Defects in CNBP are the cause of dystrophia myotonica type 2 (DM2); also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor; RNA-binding
Cellular Component: endoplasmic reticulum; cytosol; nucleus
Molecular Function: single-stranded RNA binding; zinc ion binding; single-stranded DNA binding
Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter
Research Articles on CNBP
1. findings showed that ZNF9 is ubiquitously expressed in mammalian tissues, and that its signal is not substantially modified in myotonic dystrophy type 2 muscles
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
