Product Name
CACNA2D2, siRNA
Full Product Name
CACNA2D2 siRNA (Rat)
Product Synonym Names
Voltage-dependent calcium channel subunit alpha-2/delta-2; Voltage-gated calcium channel subunit alpha-2/delta-2
Product Gene Name
CACNA2D2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8CFG6
Specificity
CACNA2D2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CACNA2D2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CACNA2D2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CACNA2D2 sirna
siRNA to inhibit CACNA2D2 expression using RNA interference
Applications Tested/Suitable for CACNA2D2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CACNA2D2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_783182.1
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NCBI GenBank Nucleotide #
NM_175592.2
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UniProt Primary Accession #
Q8CFG6
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UniProt Related Accession #
Q8CFG6[Other Products]
Molecular Weight
130,776 Da
NCBI Official Full Name
voltage-dependent calcium channel subunit alpha-2/delta-2
NCBI Official Synonym Full Names
calcium channel, voltage-dependent, alpha 2/delta subunit 2
NCBI Official Symbol
Cacna2d2 [Similar Products]
NCBI Protein Information
voltage-dependent calcium channel subunit alpha-2/delta-2
UniProt Protein Name
Voltage-dependent calcium channel subunit alpha-2/delta-2
UniProt Synonym Protein Names
Voltage-gated calcium channel subunit alpha-2/delta-2
Protein Family
Voltage-dependent calcium channel
UniProt Gene Name
Cacna2d2 [Similar Products]
UniProt Entry Name
CA2D2_RAT
NCBI Summary for CACNA2D2
auxiliary subunit of a voltage dependent calcium channel; may be involved in calcium currents in the heart [RGD, Feb 2006]
UniProt Comments for CACNA2D2
The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G). Overexpression induces apoptosis ().
Research Articles on CACNA2D2
1. 0.05), which was also higher than in the normal control group (0.137 +/- 0.013, P<0.01).">There was no difference in the mRNA expression of alpha2delta-2 (Cacna2d2) between the BRL group and CHF control group (0.264 +/- 0.005 vs. 0.243 +/- 0.017, P>0.05), which was also higher than in the normal control group (0.137 +/- 0.013, P<0.01).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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