POU1F1, Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Protein A purified

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-POU1F1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This is a rabbit polyclonal antibody against POU1F1. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP.

Polyclonal; Transcription Factor; Transcription Regulation; Transcription Regulation; Developmental Biology; DNA/RNA/Protein Interactions; Transcription Factors;

Western Blot (WB)

WB Suggested Anti-POU1F1 Antibody Titration: 1.0ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate

33kDa

POU class 1 homeobox 1

pituitary-specific positive transcription factor 1

Pituitary-specific positive transcription factor 1

GHF1; PIT1; PIT-1; GHF-1  [Similar Products]

This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

GHF-1: Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'- TAAAT-3'. Belongs to the POU transcription factor family. Class- 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 3p11.2

Cellular Component: nucleus; transcription factor complex

Molecular Function: chromatin binding; DNA binding transcription factor activity; transcription corepressor activity

Biological Process: B cell differentiation; cell fate specification; determination of ***** lifespan; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; nuclear transport; positive regulation of cell proliferation; positive regulation of inositol trisphosphate biosynthetic process; positive regulation of multicellular organism growth; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of insulin-like growth factor receptor signaling pathway; regulation of transcription, DNA-templated; somatotropin secreting cell development; transcription from RNA polymerase II promoter

Disease: Pituitary Hormone Deficiency, Combined, 1

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