Product Name
IQCB1, siRNA
Full Product Name
IQCB1 siRNA (Human)
Product Synonym Names
KIAA0036; NPHP5; IQ calmodulin-binding motif-containing protein 1; Nephrocystin-5; p53 and DNA damage-regulated IQ motif protein; PIQ
Product Gene Name
IQCB1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q15051
Specificity
IQCB1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IQCB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of IQCB1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IQCB1 sirna
siRNA to inhibit IQCB1 expression using RNA interference
Applications Tested/Suitable for IQCB1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for IQCB1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001018864.2
[Other Products]
NCBI GenBank Nucleotide #
NM_001023570.2
[Other Products]
UniProt Primary Accession #
Q15051
[Other Products]
UniProt Secondary Accession #
Q3KS08; Q3KS09; Q5DKQ7; Q8NI79; Q9BS08[Other Products]
UniProt Related Accession #
Q15051[Other Products]
Molecular Weight
34,132 Da
NCBI Official Full Name
IQ calmodulin-binding motif-containing protein 1 isoform a
NCBI Official Synonym Full Names
IQ motif containing B1
NCBI Official Symbol
IQCB1 [Similar Products]
NCBI Official Synonym Symbols
PIQ; NPHP5; SLSN5
[Similar Products]
NCBI Protein Information
IQ calmodulin-binding motif-containing protein 1
UniProt Protein Name
IQ calmodulin-binding motif-containing protein 1
UniProt Synonym Protein Names
Nephrocystin-5; p53 and DNA damage-regulated IQ motif protein; PIQ
Protein Family
IQ calmodulin-binding motif-containing protein
UniProt Gene Name
IQCB1 [Similar Products]
UniProt Synonym Gene Names
KIAA0036; NPHP5; PIQ [Similar Products]
UniProt Entry Name
IQCB1_HUMAN
NCBI Summary for IQCB1
This gene encodes a nephrocystin protein that interacts with calmodulin and the retinitis pigmentosa GTPase regulator protein. The encoded protein has a central coiled-coil region and two calmodulin-binding IQ domains. It is localized to the primary cilia of renal epithelial cells and connecting cilia of photoreceptor cells. The protein is thought to play a role in ciliary function. Defects in this gene result in Senior-Loken syndrome type 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]
UniProt Comments for IQCB1
IQCB1: Involved in ciliogenesis. Defects in IQCB1 are the cause of Senior-Loken syndrome type 5 (SLSN5). SLSN is a renal-retinal disorder, characterized by progressive wasting of the filtering unit of the kidney (nephronophthisis), with or without medullary cystic renal disease, and progressive eye disease. Typically this disorder becomes apparent during the first year of life. 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 3q13.33|3q21.1
Cellular Component: microtubule cytoskeleton; nucleoplasm; centrosome; intercellular bridge; photoreceptor outer segment; cytosol; photoreceptor connecting cilium
Molecular Function: calmodulin binding; protein binding; enzyme binding
Biological Process: organelle organization and biogenesis; photoreceptor cell maintenance; cilium biogenesis; maintenance of organ identity
Disease: Senior-loken Syndrome 5
Research Articles on IQCB1
1. mutation is predicted to introduce a new open reading frame that results in the truncation of the C-terminal 235 amino acids of nephrocystin-5 and its consequent loss of function
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
