MYH9, Polyclonal Antibody

Myosin-9; Cellular myosin heavy chain; type A; Myosin heavy chain 9; Myosin heavy chain; non-muscle IIa; Non-muscle myosin heavy chain A; NMMHC-A; Non-muscle myosin heavy chain IIa; NMMHC II-a; NMMHC-IIA; MYH9

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Ig

Rabbit

This MYH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-165 amino acids from the N-terminal region of human MYH9.

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-MYH9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.

Background: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10.

Primary Antibodies; Signal Transduction

Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)

WB:~~1:1000
FC:~~1:10~50

All lanes : Anti-MYH9 Antibody (N-term Y158) at 1:1000 dilution
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 227 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

MYH9 Antibody (N-term Y158) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the MYH9 antibody detected the MYH9 protein (arrow).

MYH9 Antibody (N-term Y158) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

226532 Da

myosin, heavy chain 9, non-muscle

myosin-9

Myosin-9

NMMHC-A; NMMHC II-a; NMMHC-IIA  [Similar Products]

MYH9_HUMAN

This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011]

MYH9: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Interacts with PDLIM2. Interacts with SLC6A4. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1. Interacts with DDR1. Interacts with SVIL and HTRA3. In the kidney, expressed in the glomeruli. Also expressed in leukocytes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: actin cytoskeleton; actomyosin; brush border; cell-cell adherens junction; cleavage furrow; contractile ring; cytoplasm; cytosol; focal adhesion; immunological synapse; integrin complex; leading edge; membrane; myosin II complex; neuromuscular junction; nucleus; plasma membrane; protein complex; ruffle; signalosome; spindle; stress fiber; uropod

Molecular Function: actin binding; actin filament binding; actin-dependent ATPase activity; ADP binding; ATP binding; ATPase activity; calmodulin binding; microfilament motor activity; motor activity; protein anchor; protein binding; protein domain specific binding; protein homodimerization activity

Biological Process: actin cytoskeleton reorganization; actin filament-based movement; actomyosin structure organization and biogenesis; angiogenesis; blood vessel endothelial cell migration; cytokinesis; establishment of meiotic spindle localization; establishment of T cell polarity; in utero embryonic development; integrin-mediated signaling pathway; leukocyte migration; meiotic spindle organization and biogenesis; membrane protein ectodomain proteolysis; monocyte differentiation; myoblast fusion; phagocytosis, engulfment; platelet formation; protein transport; regulation of cell shape; uropod organization and biogenesis

Disease: Deafness, Autosomal Dominant 17; Epstein Syndrome; Fechtner Syndrome; Macrothrombocytopenia And Progressive Sensorineural Deafness; May-hegglin Anomaly; Sebastian Syndrome

Arii, J., et al. Nature 467 (7317):859-862 (2010). Genovese, G., et al. Kidney Int. 78 (7):698-704 (2010). Tzur, S., et al. Hum. Genet. 128 (3):345-350 (2010). Bostrom, M.A., et al. Hum. Genet. 128 (2):195-204 (2010). Oleksyk, T.K., et al. PLoS ONE 5 (7), E11474 (2010).

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