CYP11A1, Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab); CYP11A; CYPXIA1; P45SCC; Cholesterol side-chain cleavage enzyme, mitochondrial; Cholesterol desmolase; Cytochrome P45 11A1; Cytochrome P45(scc); CYP11A1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CYP11A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

WB: 1:100~1:500
IHC-P: 1:100~1:500

Lane 1: mouse kidney lysates
Lane 2: human kidney lysates probed with Anti CYP11A1/P450SCC Polyclonal Antibody, Unconjugated (MBS9216556) at 1:200 in 4?C.
Followed by conjugation to secondary antibody at 1:3000 90min in 37?C.
Predicted band 53/57kD.
Observed band size: 53kD.

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti CYP11A1/P450SCC Polyclonal Antibody, Unconjugated (MBS9216556) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Mouse placenta lysates probed with Rabbit Anti-CYP11A1 Polyclonal Antibody, Unconjugated (MBS9216556) at 1:300 overnight at 4?C. Followed by conjugation to secondary antibody at 1:500 for 90 min at 37?C.

60102

Cholesterol side-chain cleavage enzyme, mitochondrial

CYP11A  [Similar Products]

CP11A_HUMAN

CYP11A1: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR). A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia. Belongs to the cytochrome P450 family.

Protein type: Mitochondrial; Oxidoreductase; EC 1.14.15.6; Lipid Metabolism - C21-steroid hormone

Chromosomal Location of Human Ortholog: 15q23-q24

Cellular Component: mitochondrial crista; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; perikaryon

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; cholesterol monooxygenase (side-chain-cleaving) activity; iron ion binding; cholesterol binding; heme binding

Biological Process: response to alkaloid; steroid metabolic process; maternal process involved in pregnancy; dibenzo-p-dioxin metabolic process; response to insecticide; response to L-ascorbic acid; estrogen biosynthetic process; response to salt stress; response to vitamin E; Leydig cell differentiation; biphenyl metabolic process; cerebellum development; response to gamma radiation; response to corticosterone stimulus; response to drug; cholesterol metabolic process; progesterone biosynthetic process; hippocampus development; phthalate metabolic process; mating behavior; response to amino acid stimulus; vitamin D metabolic process; Schwann cell differentiation; response to genistein; response to hydrogen peroxide; xenobiotic metabolic process; C21-steroid hormone biosynthetic process; glucocorticoid biosynthetic process; sterol metabolic process; granulosa cell differentiation; phenol metabolic process

Disease: Adrenal Insufficiency, Congenital, With 46,xy Sex Reversal, Partial Or Complete

CYP11A1: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR). A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia. Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; EC 1.14.15.6; Lipid Metabolism - C21-steroid hormone; Mitochondrial

Chromosomal Location of Human Ortholog: 15q23-q24

Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion

Molecular Function: cholesterol monooxygenase (side-chain-cleaving) activity; heme binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen

Biological Process: C21-steroid hormone biosynthetic process; cholesterol metabolic process; glucocorticoid biosynthetic process; steroid metabolic process; sterol metabolic process; vitamin D metabolic process; xenobiotic metabolic process

Disease: Adrenal Insufficiency, Congenital, With 46,xy Sex Reversal, Partial Or Complete

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