Aconitate hydratase, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat ACO2. No significant cross-reactivity or interference between Rat ACO2 and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ACO2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS281266 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aconitate hydratase (ACO2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACO2. The ELISA analytical biochemical technique of the MBS281266 kit is based on ACO2 antibody-ACO2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACO2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACO2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ACO2 in samples. An antibody specific for ACO2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACO2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACO2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACO2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

85,433 Da

aconitase 2

aconitate hydratase, mitochondrial

Aconitate hydratase, mitochondrial

Aconitase  [Similar Products]

ACO2: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. Monomer. Belongs to the aconitase/IPM isomerase family.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 4.2.1.3; Lyase; Mitochondrial

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: mitochondrion; myelin sheath

Molecular Function: 3 iron, 4 sulfur cluster binding; 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; iron ion binding; protein binding

Biological Process: citrate metabolic process; isocitrate metabolic process; liver development; tricarboxylic acid cycle

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