Actinin Alpha 1 (ACTN1), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of ACTN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9353989 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Actinin Alpha 1 (ACTN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACTN1. The ELISA analytical biochemical technique of the MBS9353989 kit is based on ACTN1 antibody-ACTN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACTN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACTN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

107,141 Da

actinin alpha 1

alpha-actinin-1

Alpha-actinin-1

ACTN1_HUMAN

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

ACTN1: a cytoskeletal protein of the spectrin superfamily. An actin-binding protein with multiple roles in different cell types. A nonmuscle isoform structurally similar to the erythroid beta spectrin gene.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q24|14q22-q24

Cellular Component: actin filament; brush border; cell projection; cytoplasm; cytosol; extracellular region; extracellular space; fascia adherens; focal adhesion; intercellular junction; intracellular; plasma membrane; pseudopodium; ruffle; stress fiber; Z disc

Molecular Function: actin filament binding; calcium ion binding; double-stranded RNA binding; integrin binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein homodimerization activity; vinculin binding

Biological Process: actin crosslink formation; actin filament bundle formation; actin filament network formation; actin filament organization; blood coagulation; extracellular matrix organization and biogenesis; focal adhesion formation; negative regulation of cell motility; platelet activation; platelet degranulation; platelet formation; regulation of apoptosis

Disease: Bleeding Disorder, Platelet-type, 15

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