Full Product Name
JPH3 siRNA (Human)
Product Synonym Names
JP3; TNRC22; Junctophilin-3; JP-3; Junctophilin type 3; Trinucleotide repeat-containing gene 22 protein
Product Gene Name
JPH3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8WXH2
Specificity
JPH3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human JPH3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of JPH3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
JPH3 sirna
siRNA to inhibit JPH3 expression using RNA interference
Applications Tested/Suitable for JPH3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for JPH3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_065706.2
[Other Products]
NCBI GenBank Nucleotide #
NM_020655.3
[Other Products]
UniProt Primary Accession #
Q8WXH2
[Other Products]
UniProt Secondary Accession #
Q8N471; Q9HDC3; Q9HDC4; D3DUN2[Other Products]
UniProt Related Accession #
Q8WXH2[Other Products]
Molecular Weight
19,766 Da
NCBI Official Full Name
junctophilin-3 isoform 1
NCBI Official Synonym Full Names
junctophilin 3
NCBI Official Symbol
JPH3 [Similar Products]
NCBI Official Synonym Symbols
JP3; HDL2; JP-3; TNRC22; CAGL237
[Similar Products]
NCBI Protein Information
junctophilin-3
UniProt Protein Name
Junctophilin-3
UniProt Synonym Protein Names
Junctophilin type 3; Trinucleotide repeat-containing gene 22 protein
Protein Family
Junctophilin
UniProt Gene Name
JPH3 [Similar Products]
UniProt Synonym Gene Names
JP3; TNRC22; JP-3 [Similar Products]
UniProt Entry Name
JPH3_HUMAN
NCBI Summary for JPH3
Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. CAG/CTG repeat expansions at the Huntington's disease (HD)-like 2 locus have been identified in this gene, which is a member of the junctophilin gene family. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2012]
UniProt Comments for JPH3
JPH3: Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH3 is brain- specific and appears to have an active role in certain neurons involved in motor coordination and memory. Defects in JPH3 are the cause of Huntington disease-like type 2 (HDL2). Huntington disease (HD) is a neurodegenerative disorder resulting primarily from the loss of medium spiny projection neurons in the striatum, especially in the caudate nucleus, and, to a lesser extent, atrophy of mesencephalic and cortical structures. The typical clinical picture of HD combines familial ***** onset chorea and subcortical dementia that usually begin during the fourth decade of life. Belongs to the junctophilin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Endoplasmic reticulum; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16q24.3
Cellular Component: junctional membrane complex; integral to membrane; plasma membrane
Molecular Function: calcium-release channel activity
Biological Process: learning; neuromuscular process controlling balance; locomotion; memory; regulation of neuronal synaptic plasticity
Disease: Huntington Disease-like 2
Research Articles on JPH3
1. JPH3 mutations should be considered in the differential diagnosis of early-onset dementia and hypokinetic-rigid syndromes in individuals of African descent.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
