Platelet factor 4 variant, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human PF4V1. No significant cross-reactivity or interference between Human PF4V1 and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of PF4V1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS281201 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Platelet factor 4 variant (PF4V1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PF4V1. The ELISA analytical biochemical technique of the MBS281201 kit is based on PF4V1 antibody-PF4V1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PF4V1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PF4V1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PF4V1 in samples. An antibody specific for PF4V1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPF4V1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PF4V1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PF4V1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

11,553 Da

platelet factor 4 variant 1

platelet factor 4 variant

Platelet factor 4 variant

CXCL4V1; SCYB4V1  [Similar Products]

The protein encoded by this gene is a chemokine that is highly similar to platelet factor 4. The encoded protein displays a strong antiangiogenic function and is regulated by chemokine (C-X-C motif) receptor 3. This protein also impairs tumor growth and can protect against blood-retinal barrier breakdown in diabetes patients. [provided by RefSeq, Nov 2015]

PF4V1: Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro). Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Chemokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space

Molecular Function: chemokine activity; CXCR chemokine receptor binding; heparin binding

Biological Process: G-protein coupled receptor signaling pathway; immune response; inflammatory response; leukocyte chemotaxis; platelet activation; regulation of cell proliferation; response to lipopolysaccharide

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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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