Histone Lysine Demethylase PHF8 (PHF8), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of PHF8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9346655 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Histone Lysine Demethylase PHF8 (PHF8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PHF8. The ELISA analytical biochemical technique of the MBS9346655 kit is based on PHF8 antibody-PHF8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PHF8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PHF8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

98,290 Da

PHD finger protein 8

histone lysine demethylase PHF8

Histone lysine demethylase PHF8

KIAA1111; ZNF422  [Similar Products]

PHF8_HUMAN

The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]

PHF8: a PHD finger protein with N(epsilon)-methyl lysine histone demethylase activity. Associated with mental retardation and cleft lip/palate. Its active site is closely related to those of FBXL10 and 11, all of which are selective for di- and mono-methylated lysine. In contrast, the JMJD2 demethylases are selective for tri- and di-methylated lysine.

Protein type: Nucleolus; Demethylase; EC 1.14.11.27

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: nuclear membrane; nucleolus; nucleoplasm; nucleus

Molecular Function: chromatin binding; histone demethylase activity; histone demethylase activity (H3-K36 specific); histone demethylase activity (H3-K9 specific); iron ion binding; methylated histone residue binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; protein binding; zinc ion binding

Biological Process: brain development; establishment and/or maintenance of chromatin architecture; G1/S transition of mitotic cell cycle; histone H3-K9 demethylation; mitotic cell cycle; positive regulation of transcription from RNA polymerase I promoter; positive regulation of transcription, DNA-dependent; transcription, DNA-dependent

Disease: Siderius X-linked Mental Retardation Syndrome

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