Product Name
3-oxo-5-beta-steroid 4-dehydrogenase (AKR1D1), Recombinant Protein
Full Product Name
Recombinant Rabbit 3-oxo-5-beta-steroid 4-dehydrogenase (AKR1D1)
Product Gene Name
AKR1D1 recombinant protein
[Similar Products]
Product Synonym Gene Name
AKR1D1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-326aa, full length protein
Sequence
MDLSATNHRI PLGDGNSIPI IGLGTYSEPK TTPKGSCATS VKIAIDTGYR HIDGAYIYQN EHEVGETFRE KIAEGKVRRE DIFYCGKLWA TNHDPVMVRP TLERTLKVLK LDYIDLYIIE IPMAFKPGDV VYPRDENGKW LYHKTNLCAT WEALEACKDA GLVKSLGVSN FNRQQLELLL NKPGLKHKPV CNQVECHPYF TQPKLLKFCQ QHDIIIVAYS PLGTCRNPMW VNTSLPPLLK DTLLNSLGKK YKKTAAQIVL RFNVQRGVVV IPKSFNPERI KENFQIFDFS LTEEEMKDIE ALNKNVRYVE LLMWRDHPEY PFNDEY
3D Structure
ModBase 3D Structure for Q9TV64
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of AKR1D1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AKR1D1 recombinant protein
The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction.
NCBI/Uniprot data below describe general gene information for AKR1D1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001075466.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001081997.1
[Other Products]
UniProt Primary Accession #
Q9TV64
[Other Products]
UniProt Related Accession #
Q9TV64[Other Products]
Molecular Weight
37,491 Da
NCBI Official Full Name
3-oxo-5-beta-steroid 4-dehydrogenase
NCBI Official Symbol
AKR1D1 [Similar Products]
NCBI Protein Information
3-oxo-5-beta-steroid 4-dehydrogenase
UniProt Protein Name
3-oxo-5-beta-steroid 4-dehydrogenase
UniProt Synonym Protein Names
Aldo-keto reductase family 1 member D1; Delta(4)-3-ketosteroid 5-beta-reductase; Delta(4)-3-oxosteroid 5-beta-reductase
Protein Family
3-oxo-5-beta-steroid 4-dehydrogenase
UniProt Gene Name
AKR1D1 [Similar Products]
UniProt Comments for AKR1D1
Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
