Adrenergic Receptor Kinase, beta 1, Monoclonal Antibody

Anti -Adrenergic Receptor Kinase, beta 1 (bARK1, B1 AR, ARK1 beta, G Protein Coupled Receptor Kinase 2, GPCR K2, GRK2)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 11; NC_000011.9 (67033905..67054029). Location: 11q13.1

Monoclonal

IgG2b

3G3

Mouse

Reacts with mammalian b-adrenergic receptor kinase.

Affinity Purified
Purified by Protein A affinity chromatography.

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-ADRBK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

G protein-coupled receptor kinases, a more recently discovered family, have the unique features of phosphorylating G-protein coupled receptors only when they are in their active or stimulated conformations. The range of signals acting through G protein-coupled receptors includes numerous neurotransmitters, chemoattractants, hormones, cytokines, and sensory stimuli such as photons or odorants. Structurally, the G-protein coupled receptor kinases show significant amino acid similarity to other kinases only in the centrally located catalytic domain of about 240 amino acid residues. Rapid phosphorylation of many G protein coupled receptors accompanies stimulus-driven desensitization. There are two types of kinases known to mediate these modifications: second messenger kinases and novel G protein-coupled receptor kinases. G protein-coupled receptor kinase family members include rhodopsin kinase, two isoforms of beta-adrenergic receptor kinase (betaARK), a gene cloned from the Huntington's disease locus on chromosome 4, GRK5, and several homologs from Drosophila. BetaARK1 is widely distributed throughout the mammalian central nervous system, especially at synapses, suggesting broader roles in regulating a wide variety of neurotransmitter receptors. BetaARK1 was identified as a kinase that phosphorylates betaARs in an agonist-dependent manner. Beta ARK1 has properties similar to the rhodopsin kinase in that BetaARK is also insensitive to cAMP, cGMP, and Ca2+ and does not phosphorylate histone. BetaARK is capable of phosphorylating photoexcited rhodopsin, although the betaAR is a better substrate for betaARK than rhodopsin. Both rhodopsin kinase and betaARK are inhibited by salts such as 0.1M NaCl, low concentrations of Zn (0.1-1mM), detergents such as digitonin, and polyanions such as dextransulfate and polyaspartic acid. Both kinases use ATP as the preferred substrate over GTP. In the case of betaARK, the kinase interacts with G protein beta-gamma subunits as well as with receptors, and the ternary complex of agonist bound receptor, kinase, and beta-gamma subunits is considered to be an active form for the phosphorylation of receptors. Multiple independent lines of evidence indicate that the phosphorylation of receptors by G-protein coupled receptor kinases in vivo regulates their function.

Antibodies; Abs to G Protein Receptors

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: Immunofluorescence: 3-10ug/ml
ELISA: 0.1-1ug/ml
Western Blot: 1-5ug/ml
Immunohistochemistry: 10ug/ml
Immunoprecipitation: 10ug/IP reaction

79,574 Da[Similar Products]

adrenergic, beta, receptor kinase 1

beta-adrenergic receptor kinase 1; beta-ARK-1; OTTHUMP00000235171; G-protein coupled receptor kinase 2

Beta-adrenergic receptor kinase 1

BARK; BARK1; GRK2  [Similar Products]

ARBK1_HUMAN

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]

GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.

Protein type: Kinase, protein; EC 2.7.11.15; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: G-protein coupled receptor kinase activity; protein binding; beta-adrenergic receptor kinase activity; Edg-2 lysophosphatidic acid receptor binding; alpha-2A adrenergic receptor binding; ATP binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; desensitization of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; fibroblast growth factor receptor signaling pathway; acetylcholine receptor signaling, muscarinic pathway; nerve growth factor receptor signaling pathway; heart development; peptidyl-threonine phosphorylation; signal transduction; negative regulation of striated muscle contraction; peptidyl-serine phosphorylation; negative regulation of the force of heart contraction by chemical signal; phospholipase C activation; receptor internalization; innate immune response; positive regulation of catecholamine secretion; cardiac muscle contraction

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.