Product Name
NHE3 (Slc9a3), Polyclonal Antibody
Full Product Name
NHE3 Antibody: RPE
Product Synonym Names
Slc9a3
Product Gene Name
anti-Slc9a3 antibody
[Similar Products]
Matching Pairs
Unconjugated Antibody: N/A (MBS806735)
RPE Conjugated Antibody: NHE3 (MBS806735)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P26433
Species Reactivity
Rat, Mouse
Form/Format
Affinity Purified
Concentration
1mg/mL (lot specific)
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide.
Preparation and Storage
-20 degree C
Other Notes
Small volumes of anti-Slc9a3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Slc9a3 antibody
Background Info: Detects ~84kDa
Scientific Background: Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).
Product Categories/Family for anti-Slc9a3 antibody
Transporters
Applications Tested/Suitable for anti-Slc9a3 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-Slc9a3 antibody
1:1000 (WB)
Western Blot (WB) of anti-Slc9a3 antibody
Western blot analysis of Rat kidney tissue lysates showing detection of NHE3 protein using Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400). Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400) at 1:1000.
NCBI/Uniprot data below describe general gene information for Slc9a3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036786
[Other Products]
NCBI GenBank Nucleotide #
NM_012654.1
[Other Products]
UniProt Primary Accession #
P26433
[Other Products]
UniProt Related Accession #
P26433[Other Products]
Molecular Weight
93,105 Da[Similar Products]
NCBI Official Full Name
sodium/hydrogen exchanger 3
NCBI Official Synonym Full Names
solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3
NCBI Official Symbol
Slc9a3 [Similar Products]
NCBI Official Synonym Symbols
Nhe3
[Similar Products]
NCBI Protein Information
sodium/hydrogen exchanger 3; NHE-3; Na(+)/H(+) exchanger 3; solute carrier family 9 member 3; solute carrier family 9, member 3; plasma membrane ion transport protein; solute carrier family 9 (sodium/hydrogen exchanger), member 3; Solute carrier family 9 (sodium/hydrogen exchanger 3), antiporter 3, Na+/H+ (amiloride insensitive)
UniProt Protein Name
Sodium/hydrogen exchanger 3
UniProt Synonym Protein Names
Na(+)/H(+) exchanger 3; NHE-3; Solute carrier family 9 member 3
Protein Family
Sodium/hydrogen exchanger
UniProt Gene Name
Slc9a3 [Similar Products]
UniProt Synonym Gene Names
Nhe3; NHE-3 [Similar Products]
UniProt Entry Name
SL9A3_RAT
NCBI Summary for Slc9a3
displays Na+/H+ exchanger transport activity; mediates sodium ion transport across the renal proximal tubule [RGD, Feb 2006]
UniProt Comments for Slc9a3
NHE3: Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. Binds SLC9A3R1 and SLC9A3R2. Interacts with SHANK2. Interacts with PDZD3 and interactions decrease in response to elevated calcium ion levels. Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
Protein type: Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral; Transporter
Cellular Component: brush border membrane; apical plasma membrane; integral to membrane; plasma membrane; brush border
Molecular Function: protein binding; sodium:hydrogen antiporter activity; PDZ domain binding
Biological Process: circadian rhythm; receptor-mediated endocytosis; response to glucocorticoid stimulus; sodium ion transport; regulation of pH; regulation of sodium ion transport
Product References and Citations for anti-Slc9a3 antibody
1. Kim G.H., Ecelbarger C.A., Knepper M.A. and Packer R.K. (1999) J Am Soc Nephrol. 10: 935-942. 2. Dynia D.W., Steinmetz A.G. and Kocinsky H.S. (2010) Am J Physoil Renal Physiol. 298(3): F745-F753.
Research Articles on Slc9a3
1. results indicate that central NHE3 might be a molecular target for sleep apnea treatment, whose inhibitors may be potential therapeutic drugs for sleep apnea.
Precautions
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