Runt Related Transcription Factor 2 (RUNX2), Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 17; NC_000083.6 (44495986..44814835, complement). Location: 17 B3; 17 21.33 cM

E Coli

> 95%

Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Small volumes of RUNX2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

26.5kDa

runt related transcription factor 2

runt-related transcription factor 2; CBF-alpha-1; PEA2-alpha A; PEBP2 alpha A; AKV core binding factor; core binding factor alpha 1; runt domain, alpha subunit 1; acute myeloid leukemia 3 protein; SL3-3 enhancer factor 1 alpha A subunit; osteoblast-specific transcription factor 2; polyomavirus enhancer-binding protein 2 alpha A subunit

Runt-related transcription factor 2

Aml3; Cbfa1; Osf2; Pebp2a; CBF-alpha-1; OSF-2; PEA2-alpha A  [Similar Products]

RUNX2_MOUSE

AML3: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors. Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 and HIPK3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with KAT6A and KAT6B. Binds to cyclin B1 CCNB1. Interacts with DDX5. Specifically expressed in osteoblasts. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; nucleus

Molecular Function: protein domain specific binding; protein binding; DNA binding; histone deacetylase binding; bHLH transcription factor binding; chromatin binding; transcription factor binding; transcription factor activity; ATP binding

Biological Process: embryonic forelimb morphogenesis; transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; cell maturation; BMP signaling pathway; neuron differentiation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; chondrocyte differentiation; skeletal development; regulation of odontogenesis of dentine-containing teeth; T cell differentiation; endochondral ossification; transcription, DNA-dependent; regulation of osteoblast differentiation; regulation of fibroblast growth factor receptor signaling pathway; embryonic cranial skeleton morphogenesis; stem cell differentiation; chondrocyte development; positive regulation of ossification; osteoblast development; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; skeletal morphogenesis; negative regulation of smoothened signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of ossification; negative regulation of transcription, DNA-dependent; osteoblast fate commitment

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