SOX2, Polyclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Affinity Purified

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-SOX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This is a rabbit polyclonal antibody against SOX2. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in this gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation. This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Polyclonal; Transcription Factor; Transcription Regulation; Transcription Regulation; Developmental Biology; Stem Cells; Disease Related; Immunohistochemistry; Transcription Factors;

Immunohistochemistry (IHC), Western Blot (WB)

Human Kidney

Human Spleen

Researcher:Kim Perry, University of Illinois
Application:IHC
Species+tissue/cell type:Xenopus laevis cornea epithellium
Primary antibody dilution:1:300
Secondary antibody:Goat anti-rabbit -rhodamine
Secondary antibody dilution:1:300

Host: Mouse
Target Name: SOX2
Sample Tissue: Mouse Liver
Antibody Dilution: 1ug/ml

Host: Mouse
Target Name: SOX2
Sample Tissue: Mouse Pancreas
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: SOX2
Sample Tissue: Human 293T
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: SOX2
Sample Tissue: Mouse Liver
Antibody Dilution: 1ug/ml

Sample Type: Lane 1: 20 ug U87 lysate
Primary Antibody Dilution: 1:1000
Secondary Antibody: Anti-rabbit-HRP
Secondary Antibody Dilution: 1:2000
Submitted by: Ander Matheu Fernandez, Biodonostia Institute

WB Suggested Anti-SOX2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:500
Positive Control: OVCAR-3 cell lysateThere is BioGPS gene expression data showing that SOX2 is expressed in OVCAR3

34kDa

SRY-box 2

transcription factor SOX-2

Transcription factor SOX-2

SOX2_HUMAN

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]

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