MRE11, Polyclonal Antibody

MRE11A; HNGS1; MRE11; Double-strand break repair protein MRE11A; Meiotic recombination 11 homolog 1; MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Phospho-MRE11 (S264) Polyclonal Antibody detects endogenous levels of MRE11 protein only when phosphorylated at S264.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

1 mg/ml (lot specific)

Store at -20 degree C/1 year

Small volumes of anti-MRE11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Phospho-specific Ab

Western Blot (WB), ELISA (EIA)

Western blotting: 1:500 - 1:2000
ELISA: 1:5000
Not yet tested in other applications

Western Blot analysis of 3T3 cells using Phospho-MRE11 (S264) Polyclonal Antibody

81,070 Da

MRE11 homolog A, double strand break repair nuclease

double-strand break repair protein MRE11A

Double-strand break repair protein MRE11A

HNGS1; MRE11; MRE11 homolog 1; MRE11 homolog A  [Similar Products]

MRE11_HUMAN

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

MRE11A: a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Alternative splicing results in two different isoforms.

Protein type: DNA-binding; Deoxyribonuclease; Cell cycle regulation; DNA repair, damage

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: nucleoplasm; chromosome, telomeric region; Mre11 complex; chromatin; cytosol; nucleus

Molecular Function: protein C-terminus binding; ATP-dependent DNA helicase activity; protein binding; nuclease activity; DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; manganese ion binding; endonuclease activity; double-stranded DNA binding; 3'-5' exonuclease activity; endodeoxyribonuclease activity

Biological Process: sister chromatid cohesion; positive regulation of kinase activity; negative regulation of DNA endoreduplication; telomere maintenance via telomerase; regulation of mitotic recombination; DNA repair; DNA catabolic process, endonucleolytic; positive regulation of protein amino acid autophosphorylation; double-strand break repair via nonhomologous end joining; DNA duplex unwinding; double-strand break repair via homologous recombination; DNA recombination; cell proliferation; nucleotide-excision repair; intra-S DNA damage checkpoint; base-excision repair; double-strand break repair; mitotic cell cycle G2/M transition DNA damage checkpoint; meiotic recombination; synapsis; positive regulation of interferon type I production; innate immune response; response to DNA damage stimulus; telomere maintenance

Disease: Ataxia-telangiectasia-like Disorder 1

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