Glucosidase Alpha, Acid (GaA), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of GaA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9136957 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glucosidase Alpha, Acid (GaA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GaA. The ELISA analytical biochemical technique of the MBS9136957 kit is based on GaA antibody-GaA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GaA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GaA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Product Overview: The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
Intended Use: This Human Glucosidase Alpha, Acid (GaA) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human Glucosidase Alpha, Acid (GaA) in the sample, this Human Glucosidase Alpha, Acid (GaA) ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human Glucosidase Alpha, Acid (GaA) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.

105,324 Da

glucosidase alpha, acid

lysosomal alpha-glucosidase

Lysosomal alpha-glucosidase

This gene encodes lysosomal alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. The encoded preproprotein is proteolytically processed to generate multiple intermediate forms and the mature form of the enzyme. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Essential for the degradation of glycogen in lysosomes (PubMed:1856189, PubMed:7717400, PubMed:14695532, PubMed:18429042). Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans (PubMed:29061980).

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