AP2S1, Polyclonal Antibody

AP-2 complex subunit sigma; Adaptor protein complex AP-2 subunit sigma; Adaptor-related protein complex 2 subunit sigma; Clathrin assembly protein 2 sigma small chain; Clathrin coat assembly protein AP17; Clathrin coat-associated protein AP17; HA2 17 kDa subunit; Plasma membrane adaptor AP-2 17 kDa protein; Sigma2-adaptin; AP2S1; AP17; CLAPS2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This AP2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-64 amino acids from the Central region of human AP2S1.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.39 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-AP2S1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

One of two major clathrin-associated adaptor complexes,
AP-2, is a heterotetramer which is associated with the plasma
membrane. This complex is composed of two large chains, a medium
chain, and a small chain. This gene encodes the small chain of this
complex. Alternative splicing has been observed in this gene and
results in two known transcripts.

Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:1000

AP2S1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the AP2S1 antibody detected the AP2S1 protein (arrow).

17018

adaptor-related protein complex 2, sigma 1 subunit

AP-2 complex subunit sigma

AP-2 complex subunit sigma

AP17; CLAPS2  [Similar Products]

AP2S1_HUMAN

One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

AP2S1: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Belongs to the adaptor complexes small subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: plasma membrane; AP-2 adaptor complex; cytosol

Molecular Function: transporter activity; protein transporter activity

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; intracellular protein transport; synaptic transmission; clathrin cage assembly; axon guidance; viral reproduction; nerve growth factor receptor signaling pathway; regulation of endocytosis; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus

Disease: Hypocalciuric Hypercalcemia, Familial, Type Iii

Stove, V., et al. J. Virol. 79(17):11422-11433(2005)
Lu, W., et al. Neuron 47(3):407-421(2005)
Collins, B.M., et al. Cell 109(4):523-535(2002)
Yano, H., et al. J. Neurosci. 21 (3), RC125 (2001) :
Pearse, B.M., et al. Curr. Opin. Struct. Biol. 10(2):220-228(2000)

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